TY - JOUR
T1 - Recent Advances of CRISPR/Cas9-Based Genetic Engineering and Transcriptional Regulation in Industrial Biology
AU - Zhang, Shangjie
AU - Guo, Feng
AU - Yan, Wei
AU - Dai, Zhongxue
AU - Dong, Weiliang
AU - Zhou, Jie
AU - Zhang, Wenming
AU - Xin, Fengxue
AU - Jiang, Min
N1 - Publisher Copyright:
© Copyright © 2020 Zhang, Guo, Yan, Dai, Dong, Zhou, Zhang, Xin and Jiang.
PY - 2020/1/28
Y1 - 2020/1/28
N2 - Industrial biology plays a crucial role in the fields of medicine, health, food, energy, and so on. However, the lack of efficient genetic engineering tools has restricted the rapid development of industrial biology. Recently, the emergence of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system brought a breakthrough in genome editing technologies due to its high orthogonality, versatility, and efficiency. In this review, we summarized the barriers of CRISPR/Cas9 and corresponding solutions for efficient genetic engineering in industrial microorganisms. In addition, the advances of industrial biology employing the CRISPR/Cas9 system were compared in terms of its application in bacteria, yeast, and filamentous fungi. Furthermore, the cooperation between CRISPR/Cas9 and synthetic biology was discussed to help build complex and programmable gene circuits, which can be used in industrial biotechnology.
AB - Industrial biology plays a crucial role in the fields of medicine, health, food, energy, and so on. However, the lack of efficient genetic engineering tools has restricted the rapid development of industrial biology. Recently, the emergence of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system brought a breakthrough in genome editing technologies due to its high orthogonality, versatility, and efficiency. In this review, we summarized the barriers of CRISPR/Cas9 and corresponding solutions for efficient genetic engineering in industrial microorganisms. In addition, the advances of industrial biology employing the CRISPR/Cas9 system were compared in terms of its application in bacteria, yeast, and filamentous fungi. Furthermore, the cooperation between CRISPR/Cas9 and synthetic biology was discussed to help build complex and programmable gene circuits, which can be used in industrial biotechnology.
KW - CRISPR/Cas9
KW - gene circuits
KW - genetic engineering
KW - industrial biology
KW - synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85079506741&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2019.00459
DO - 10.3389/fbioe.2019.00459
M3 - 文献综述
AN - SCOPUS:85079506741
SN - 2296-4185
VL - 7
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 459
ER -