TY - JOUR
T1 - Recombinant aspartate aminotransferase-catalyzed synthesis of L-4-fluorophenylalanine
AU - Chen, Meijuan
AU - Jia, Honghua
AU - Chen, Yongsheng
AU - Jiang, Min
AU - Wei, Ping
PY - 2008/6
Y1 - 2008/6
N2 - L-4-Fluorophenylalanine (FPhe) was prepared from 4-fluorophenylpyruvate (FPPA) catalyzed by aspartate aminotransferase (Asp-AT) of the recombinant E. coli BL21-pET/aspC. After 12 h enzymatic reaction, the FPPA conversion was over 90% and the yield of FPhe could be above 85% under the following optimal conditions: 37°C, pH value range of 5.0-8.0, 5.5 mass ratio of cell to FPPA, 0.6% (w/v) of Tween 80, 7.08 g/L FPPA, and 1.6 of molar ratio of L-Asp to FPPA.
AB - L-4-Fluorophenylalanine (FPhe) was prepared from 4-fluorophenylpyruvate (FPPA) catalyzed by aspartate aminotransferase (Asp-AT) of the recombinant E. coli BL21-pET/aspC. After 12 h enzymatic reaction, the FPPA conversion was over 90% and the yield of FPhe could be above 85% under the following optimal conditions: 37°C, pH value range of 5.0-8.0, 5.5 mass ratio of cell to FPPA, 0.6% (w/v) of Tween 80, 7.08 g/L FPPA, and 1.6 of molar ratio of L-Asp to FPPA.
KW - 4-fluorophenylpyruvate
KW - Aspartate aminotransferase
KW - L-4-fluorophenylalanine
UR - http://www.scopus.com/inward/record.url?scp=44949101338&partnerID=8YFLogxK
U2 - 10.1007/s11705-008-0041-0
DO - 10.1007/s11705-008-0041-0
M3 - 文章
AN - SCOPUS:44949101338
SN - 1673-7369
VL - 2
SP - 220
EP - 223
JO - Frontiers of Chemical Engineering in China
JF - Frontiers of Chemical Engineering in China
IS - 2
ER -