[Screening and breeding high 1,3-propanediol producing strains by genome shuffling].

To improve the tolerance of main metabolites, we used genome shuffling to achieve high 1,3-propanediol producing mutants. Based on 96 deep-well palates containing prepared ended fed-batch broth as an efficient selection method, genome shuffling has been applied in strain improvement. Five high producers were obtained after genome shuffling (LSG1, LSG2, LSG4, LSG5 and LSG6). During batch fermentation (3 L), the 1, 3-propanediol production of the five mutants were improved 17.0%, 19.0%, 12.9%, 23.9% and 18.0% , compared with the parent strain; the conservations from glycerol were improved 17.7%, 20.0%, 13.3%, 24.4% and 17.7%. Genome shuffling was an efficient approach for strain improvement, and 96 deep-well palates containing fed-batch broth has been demonstrated as an efficient selection approach.