Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational design

Bo Fan; Wenxin Dong; Tianyi Chen; Jianlin Chu; Bingfang He

doi:10.1039/c8ob00376a

Switching glycosyltransferase UGT_BL1 regioselectivity toward polydatin synthesis using a semi-rational design

Bo Fan, Wenxin Dong, Tianyi Chen, Jianlin Chu, Bingfang He

药学院

科研成果: 期刊稿件 › 文章 › 同行评审

24 引用（Scopus）

摘要

The 62nd residue of glycosyltransferase UGT_BL1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product.

源语言	英语
页（从-至）	2464-2469
页数	6
期刊	Organic and Biomolecular Chemistry
卷	16
期	14
DOI	https://doi.org/10.1039/c8ob00376a
出版状态	已出版 - 2018

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AU - Dong, Wenxin

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AU - Chu, Jianlin

AU - He, Bingfang

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N2 - The 62nd residue of glycosyltransferase UGTBL1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product.

AB - The 62nd residue of glycosyltransferase UGTBL1 was identified as a "hotspot" for glycosylation at 3-OH of resveratrol. Via semi-rational design including structure-guided alanine scanning and saturation mutations, the mutation I62G significantly switched the regioselectivity from 4′-OH to 3-OH of resveratrol and mainly produced polydatin (87.7%), a therapeutic natural product.

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Switching glycosyltransferase UGTBL1 regioselectivity toward polydatin synthesis using a semi-rational design

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Switching glycosyltransferase UGT_BL1 regioselectivity toward polydatin synthesis using a semi-rational design