TY - JOUR
T1 - Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization
AU - Suo, Hongbo
AU - Gao, Zhen
AU - Xu, Lili
AU - Xu, Chao
AU - Yu, Dinghua
AU - Xiang, Xinran
AU - Huang, He
AU - Hu, Yi
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3
Y1 - 2019/3
N2 - We developed magnetic chitosan nanoparticles (CS‑Fe 3 O 4 ) with mean diameter of 15–20 nm. Subsequently, these inorganic-organic composite nanoparticles were modified using an imidazole-based functional ionic liquid (IL). The prepared support (IL‑CS‑Fe 3 O 4 ), which was used to immobilize porcine pancreatic lipase (PPL), was characterized using Fourier transform infrared (FTIR) spectroscopy, vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and X-ray diffraction (XRD). Circular dichroism (CD) was used to analyze the secondary structure of immobilized PPL. The immobilized PPL (PPL‑IL‑CS‑Fe 3 O 4 ) exhibited 1.93-fold higher specific activity than PPL‑CS-Fe 3 O 4 when triacetin was used as the substrate, and showed 95 mg/g of lipase immobilization capacity and 382% of activity recovery. The residual activity of PPL‑IL‑CS‑Fe 3 O 4 was above 60% of the initial activity after incubation at 50 °C for 6 h, as was higher than that of PPL‑CS‑Fe 3 O 4 which showed 40% of the initial activity. In addition, PPL‑IL‑CS‑Fe 3 O 4 retained 84.6% of the initial activity after 10 cycles, whereas PPL‑CS‑Fe 3 O 4 retained only 75.5% activity. Furthermore, the kinetic parameters, apparent K m and V max of PPL‑IL‑CS‑Fe 3 O 4 were 2.51 mg/mL and 1.395 U/mg respectively, these results indicated that the immobilized PPL had better affinity towards the substrate, especially when the nanoparticles were modified by functional IL. Besides, the magnetic chitosan nanoparticles loaded with PPL were easily recovered. A novel, efficient, and practical method for enzyme immobilization was developed.
AB - We developed magnetic chitosan nanoparticles (CS‑Fe 3 O 4 ) with mean diameter of 15–20 nm. Subsequently, these inorganic-organic composite nanoparticles were modified using an imidazole-based functional ionic liquid (IL). The prepared support (IL‑CS‑Fe 3 O 4 ), which was used to immobilize porcine pancreatic lipase (PPL), was characterized using Fourier transform infrared (FTIR) spectroscopy, vibrating sample magnetometry (VSM), thermogravimetry (TG), transmission electron microscopy (TEM) and X-ray diffraction (XRD). Circular dichroism (CD) was used to analyze the secondary structure of immobilized PPL. The immobilized PPL (PPL‑IL‑CS‑Fe 3 O 4 ) exhibited 1.93-fold higher specific activity than PPL‑CS-Fe 3 O 4 when triacetin was used as the substrate, and showed 95 mg/g of lipase immobilization capacity and 382% of activity recovery. The residual activity of PPL‑IL‑CS‑Fe 3 O 4 was above 60% of the initial activity after incubation at 50 °C for 6 h, as was higher than that of PPL‑CS‑Fe 3 O 4 which showed 40% of the initial activity. In addition, PPL‑IL‑CS‑Fe 3 O 4 retained 84.6% of the initial activity after 10 cycles, whereas PPL‑CS‑Fe 3 O 4 retained only 75.5% activity. Furthermore, the kinetic parameters, apparent K m and V max of PPL‑IL‑CS‑Fe 3 O 4 were 2.51 mg/mL and 1.395 U/mg respectively, these results indicated that the immobilized PPL had better affinity towards the substrate, especially when the nanoparticles were modified by functional IL. Besides, the magnetic chitosan nanoparticles loaded with PPL were easily recovered. A novel, efficient, and practical method for enzyme immobilization was developed.
KW - Composite nanoparticles
KW - Enzyme immobilization
KW - Ionic liquid
KW - Porcine pancreatic lipase
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=85057260152&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2018.11.041
DO - 10.1016/j.msec.2018.11.041
M3 - 文章
C2 - 30606543
AN - SCOPUS:85057260152
SN - 0928-4931
VL - 96
SP - 356
EP - 364
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -