ε-Poly-L-lysine production by immobilized cells of Kitasatospora sp. MY 5-36 in repeated fed-batch cultures

Yang Zhang, Xiaohai Feng, Hong Xu, Zhong Yao, Pingkai Ouyang

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

The production of ε-PL by Kitasatospora sp. MY 5-36 through entrapment or adsorption on bagasse, synthetic sponge, macroporous silica gel, and loofah sponge was investigated in shake flask cultures, and immobilization on loofah sponge gave the highest ε-PL production. Repeated fed-batch cultures for ε-PL production were also carried out in a stirred bioreactor and final ε-PL concentrations and productivity of 34.11 g L-1 and 9.34 g L-1 d-1, respectively were achieved by cells immobilize in loofah sponge. These values exceeded those for cultures with free cells (22.53 g L-1 and 3.30 g L-1 d-1). The immobilized cells were reused five times over a period of 526 h. These results suggest that the immobilization approach is promising for industrial applications.

Original languageEnglish
Pages (from-to)5523-5527
Number of pages5
JournalBioresource Technology
Volume101
Issue number14
DOIs
StatePublished - Jul 2010

Keywords

  • Immobilized cells
  • Kitasatospora sp. MY 5-36
  • Loofah sponge
  • Poly-ε-lysine
  • Repeated fed-batch

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