Abstract
The production of ε-PL by Kitasatospora sp. MY 5-36 through entrapment or adsorption on bagasse, synthetic sponge, macroporous silica gel, and loofah sponge was investigated in shake flask cultures, and immobilization on loofah sponge gave the highest ε-PL production. Repeated fed-batch cultures for ε-PL production were also carried out in a stirred bioreactor and final ε-PL concentrations and productivity of 34.11 g L-1 and 9.34 g L-1 d-1, respectively were achieved by cells immobilize in loofah sponge. These values exceeded those for cultures with free cells (22.53 g L-1 and 3.30 g L-1 d-1). The immobilized cells were reused five times over a period of 526 h. These results suggest that the immobilization approach is promising for industrial applications.
Original language | English |
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Pages (from-to) | 5523-5527 |
Number of pages | 5 |
Journal | Bioresource Technology |
Volume | 101 |
Issue number | 14 |
DOIs | |
State | Published - Jul 2010 |
Keywords
- Immobilized cells
- Kitasatospora sp. MY 5-36
- Loofah sponge
- Poly-ε-lysine
- Repeated fed-batch