蛹虫草发酵产虫草素工艺优化

In order to determine the amplification technology for cordycepin, the effects of cultivation quality and height-diameter ratio of fermentation on the synthesis of cordycepin were investigated. The results showed that the concentration of cordycepin reached 6 g/L when the optimal conditions of seed cultivation were as follows: the rotation speed of 250 r/min, the inoculum size of 15%, and the subculture times of two. In addition, the optimal height-diameter ratio of liquid loading was 2 cm/30 cm. Under these conditions, a 120 L multi-layer reactor was used to verify the fermentation process. After 25 days of fermentation, the concentration of cordycepin was 5.32 g/L and the conversion rate was 57%, which was consistent with the results of small scale test. The experimental results showed that the process scaling up is feasible, which provides a reference for further industrialization.