A chemiluminescent probe for the real-time monitoring of esterases activities

Fan Wang; Yuanbo Wang; Jiawei Zhang; Shiyue Zheng; Bin Xie; Sheng Lu; Jie Zhou; Chongqing Wang; Fang Wang; Min Jiang; Xiaoqiang Chen

doi:10.1016/j.snb.2022.132880

A chemiluminescent probe for the real-time monitoring of esterases activities

Fan Wang, Yuanbo Wang, Jiawei Zhang, Shiyue Zheng, Bin Xie, Sheng Lu, Jie Zhou, Chongqing Wang, Fang Wang, Min Jiang, Xiaoqiang Chen

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Esterases are common (biological catalyst) that play an essential role in regulating the physiological processes of organisms, in addition to contributing to disease prevention. Herein, we report the design and synthesis of an esterase-activated chemiluminescent probe (CL-EA) for the selective detection of esterases. Using the 1,2-dioxetane scaffold as the chemiluminescent platform and acetoxymethyl ether as the recognition group, the present probe was successfully lighted in the presence of esterases. As a result, a strong chemiluminescent signal was generated in an aqueous solution, thereby allowing the highly sensitive and specific detection of esterases. Furthermore, the probe is used to screen the activity of three plastic-degrading enzymes and image esterases in live cells and in a mouse model.

Original language	English
Article number	132880
Journal	Sensors and Actuators B: Chemical
Volume	375
DOIs	https://doi.org/10.1016/j.snb.2022.132880
State	Published - 15 Jan 2023

Keywords

1,2-Dioxetane
Bio-imaging
Chemiluminescent probe
Esterases

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10.1016/j.snb.2022.132880

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title = "A chemiluminescent probe for the real-time monitoring of esterases activities",

abstract = "Esterases are common (biological catalyst) that play an essential role in regulating the physiological processes of organisms, in addition to contributing to disease prevention. Herein, we report the design and synthesis of an esterase-activated chemiluminescent probe (CL-EA) for the selective detection of esterases. Using the 1,2-dioxetane scaffold as the chemiluminescent platform and acetoxymethyl ether as the recognition group, the present probe was successfully lighted in the presence of esterases. As a result, a strong chemiluminescent signal was generated in an aqueous solution, thereby allowing the highly sensitive and specific detection of esterases. Furthermore, the probe is used to screen the activity of three plastic-degrading enzymes and image esterases in live cells and in a mouse model.",

keywords = "1,2-Dioxetane, Bio-imaging, Chemiluminescent probe, Esterases",

author = "Fan Wang and Yuanbo Wang and Jiawei Zhang and Shiyue Zheng and Bin Xie and Sheng Lu and Jie Zhou and Chongqing Wang and Fang Wang and Min Jiang and Xiaoqiang Chen",

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doi = "10.1016/j.snb.2022.132880",

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T1 - A chemiluminescent probe for the real-time monitoring of esterases activities

AU - Wang, Fan

AU - Wang, Yuanbo

AU - Zhang, Jiawei

AU - Zheng, Shiyue

AU - Xie, Bin

AU - Lu, Sheng

AU - Zhou, Jie

AU - Wang, Chongqing

AU - Wang, Fang

AU - Jiang, Min

AU - Chen, Xiaoqiang

PY - 2023/1/15

Y1 - 2023/1/15

N2 - Esterases are common (biological catalyst) that play an essential role in regulating the physiological processes of organisms, in addition to contributing to disease prevention. Herein, we report the design and synthesis of an esterase-activated chemiluminescent probe (CL-EA) for the selective detection of esterases. Using the 1,2-dioxetane scaffold as the chemiluminescent platform and acetoxymethyl ether as the recognition group, the present probe was successfully lighted in the presence of esterases. As a result, a strong chemiluminescent signal was generated in an aqueous solution, thereby allowing the highly sensitive and specific detection of esterases. Furthermore, the probe is used to screen the activity of three plastic-degrading enzymes and image esterases in live cells and in a mouse model.

AB - Esterases are common (biological catalyst) that play an essential role in regulating the physiological processes of organisms, in addition to contributing to disease prevention. Herein, we report the design and synthesis of an esterase-activated chemiluminescent probe (CL-EA) for the selective detection of esterases. Using the 1,2-dioxetane scaffold as the chemiluminescent platform and acetoxymethyl ether as the recognition group, the present probe was successfully lighted in the presence of esterases. As a result, a strong chemiluminescent signal was generated in an aqueous solution, thereby allowing the highly sensitive and specific detection of esterases. Furthermore, the probe is used to screen the activity of three plastic-degrading enzymes and image esterases in live cells and in a mouse model.

KW - 1,2-Dioxetane

KW - Bio-imaging

KW - Chemiluminescent probe

KW - Esterases

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U2 - 10.1016/j.snb.2022.132880

DO - 10.1016/j.snb.2022.132880

M3 - 文章

AN - SCOPUS:85140389989

SN - 0925-4005

VL - 375

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

M1 - 132880

ER -

A chemiluminescent probe for the real-time monitoring of esterases activities

Abstract

Keywords

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