TY - JOUR
T1 - A paper disk equipped with graphene/polyaniline/Au nanoparticles/glucose oxidase biocomposite modified screen-printed electrode
T2 - Toward whole blood glucose determination
AU - Kong, Fen Ying
AU - Gu, Sai Xi
AU - Li, Wei Wei
AU - Chen, Ting Ting
AU - Xu, Qin
AU - Wang, Wei
PY - 2014/6/15
Y1 - 2014/6/15
N2 - In this work, a convenient, fast, low cost, small sample volume and in situ detection of glucose in human whole blood has been developed by using a disposable screen-printed carbon electrode (SPCE) coupled with a paper disk. To perform the assay, the SPCE was modified with graphene/polyaniline/Au nanoparticles/glucose oxidase (Gr/PANI/AuNPs/GOD) biocomposite and then covered by a paper disk impregnated with the sample. After introducing PBS on the paper disk, the electrochemical measurement was carried out. The assay was based on measuring the current decrease of flavin adenine dinucleotide (FAD) in GOD provoked by the enzyme-substrate reaction using differential pulse voltammetry (DPV). The analytical performance was comparable to conventional methods, and covered the full range of clinically relevant concentrations of glucose in whole blood. This new paper-based electrochemical glucose sensor shows promise in applying point-of-care (POC) device in whole blood tests, and particularly being appropriate for use in the developing world and in resource-limited settings.
AB - In this work, a convenient, fast, low cost, small sample volume and in situ detection of glucose in human whole blood has been developed by using a disposable screen-printed carbon electrode (SPCE) coupled with a paper disk. To perform the assay, the SPCE was modified with graphene/polyaniline/Au nanoparticles/glucose oxidase (Gr/PANI/AuNPs/GOD) biocomposite and then covered by a paper disk impregnated with the sample. After introducing PBS on the paper disk, the electrochemical measurement was carried out. The assay was based on measuring the current decrease of flavin adenine dinucleotide (FAD) in GOD provoked by the enzyme-substrate reaction using differential pulse voltammetry (DPV). The analytical performance was comparable to conventional methods, and covered the full range of clinically relevant concentrations of glucose in whole blood. This new paper-based electrochemical glucose sensor shows promise in applying point-of-care (POC) device in whole blood tests, and particularly being appropriate for use in the developing world and in resource-limited settings.
KW - Electrochemical detection
KW - Glucose
KW - Paper disk
KW - Screen-printed carbon electrodes
KW - Whole blood
UR - http://www.scopus.com/inward/record.url?scp=84892972228&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2013.12.067
DO - 10.1016/j.bios.2013.12.067
M3 - 文章
C2 - 24469540
AN - SCOPUS:84892972228
SN - 0956-5663
VL - 56
SP - 77
EP - 82
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -