TY - JOUR
T1 - A Procedure to Design One-Pot Multi-enzyme System for Industrial CDP-Choline Production
AU - Zheng, Cheng
AU - Miao, Rongxin
AU - Liu, Yingmiao
AU - Cao, Yang
AU - Liu, Dong
AU - Wang, Junzhi
AU - Ying, Hanjie
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/9
Y1 - 2021/9
N2 - Fermentation and chemical methods for industrial cytidine diphosphate choline (CDP-choline) catalytic production both suffer from several disadvantages such as relatively low efficiency and productivity. To overcome these problems, we applied the concept of synthetic biology to develop a new one-pot multi-enzyme system to produce CDP-choline from orotic acid. Enzymes from different sources were selected and optimized as building blocks of the system, and parameters such as oxygen supply were also optimized. This system shows a titer of 37.6 ± 1.1 mM and a reaction rate of 1.6 mM L-1 h−1, both increase 66 % from traditional processes. It also has an efficiency of energy of 25.4%, improves 2-folds. This new one-pot CDP-choline-producing system has a potential for industrial use, and the procedure to design one-pot multi-enzyme system can be applied to build other one-pot system producing energy-rich compounds.
AB - Fermentation and chemical methods for industrial cytidine diphosphate choline (CDP-choline) catalytic production both suffer from several disadvantages such as relatively low efficiency and productivity. To overcome these problems, we applied the concept of synthetic biology to develop a new one-pot multi-enzyme system to produce CDP-choline from orotic acid. Enzymes from different sources were selected and optimized as building blocks of the system, and parameters such as oxygen supply were also optimized. This system shows a titer of 37.6 ± 1.1 mM and a reaction rate of 1.6 mM L-1 h−1, both increase 66 % from traditional processes. It also has an efficiency of energy of 25.4%, improves 2-folds. This new one-pot CDP-choline-producing system has a potential for industrial use, and the procedure to design one-pot multi-enzyme system can be applied to build other one-pot system producing energy-rich compounds.
KW - ATP regeneration
KW - CDP-choline production
KW - One-pot multi-enzyme system
KW - Oxygen supply
KW - Synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85107731221&partnerID=8YFLogxK
U2 - 10.1007/s12010-021-03564-2
DO - 10.1007/s12010-021-03564-2
M3 - 文章
C2 - 34117628
AN - SCOPUS:85107731221
SN - 0273-2289
VL - 193
SP - 2769
EP - 2780
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 9
ER -