Biocatalysts used for multi-step reactions in continuous flow

Biocatalysts toward multi-step reactions, though they make the construction of versatile molecules possible, struggle to achieve a rapid expansion due to many challenges to be addressed. Continuous flow technique has been used extensively in synthetic organic chemistry over the last decades, and the development of biocatalytic processes in continuous flow, however, has been only recently become increasingly popular. The integration of continuous flow with biocatalysts toward multi-step reactions was endowed with distinguished advantages that cannot be achieved in traditional batch mode. Within this mini-review, we will mainly summarize the recent applications in the area of biocatalysts toward multi-step continuous flow reactions, covering chemo-enzymatic and multi-enzymatic systems, with particular focus on the main challenges and strategies.