TY - JOUR
T1 - Biomass composition, lipid characterization, and metabolic profile analysis of the fed-batch fermentation process of two different docosahexanoic acid producing schizochytrium sp. strains
AU - Qu, Liang
AU - Ren, Lu Jing
AU - Li, Juan
AU - Sun, Guan Nan
AU - Sun, Li Na
AU - Ji, Xiao Jun
AU - Nie, Zhi Kui
AU - Huang, He
PY - 2013/12
Y1 - 2013/12
N2 - Growth and fermentation characteristics, biomass composition, lipid characterization and metabolic profiling analysis of two different Schizochytrium sp. strains, the original strain and the industrial adaptive strain, were investigated in the fed-batch fermentation process. The final cell biomass, total lipids content, docosahexanoic acid (DHA) content and DHA productivity of the adaptive strain were much higher than those of the original strain. The metabolic distinctions which extensively existed between these two strains were revealed by the score plot of principal component analysis. In addition, potential biomarkers responsible for discriminating different strains were identified as myo-inositol, histidine, alanine, asparagine, cysteine, and oxalic acid. These findings provided new insights into the industrial strain screening and further improvement of DHA production by Schizochytrium sp.
AB - Growth and fermentation characteristics, biomass composition, lipid characterization and metabolic profiling analysis of two different Schizochytrium sp. strains, the original strain and the industrial adaptive strain, were investigated in the fed-batch fermentation process. The final cell biomass, total lipids content, docosahexanoic acid (DHA) content and DHA productivity of the adaptive strain were much higher than those of the original strain. The metabolic distinctions which extensively existed between these two strains were revealed by the score plot of principal component analysis. In addition, potential biomarkers responsible for discriminating different strains were identified as myo-inositol, histidine, alanine, asparagine, cysteine, and oxalic acid. These findings provided new insights into the industrial strain screening and further improvement of DHA production by Schizochytrium sp.
KW - Biomass composition
KW - Docosahexanoic acid
KW - Fed-batch
KW - Lipid characterization
KW - Metabolic profiling analysis
KW - Schizochytrium sp.
UR - http://www.scopus.com/inward/record.url?scp=84890557661&partnerID=8YFLogxK
U2 - 10.1007/s12010-013-0456-z
DO - 10.1007/s12010-013-0456-z
M3 - 文章
C2 - 24061873
AN - SCOPUS:84890557661
SN - 0273-2289
VL - 171
SP - 1865
EP - 1876
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 7
ER -