Direct electron transfer of cytochrome c immobilized on a NaY zeolite matrix and its application in biosensing

Zhihui Dai, Songqin Liu, Huangxian Ju

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

The immobilization and electrochemical behaviors of cytochrome c on a NaY zeolite modified electrode were studied. The interaction between cytochrome c and NaY zeolite particles was examined by using UV-Vis spectroscopy and electrochemical methods. The direct electron transfer of the immobilized cytochrome c exhibited a pair of redox peaks with the E1/2 of -(44±3)mV (versus SCE) in 0.1M pH 7.0 PBS. The electrode reaction showed a surface-controlled process with a single proton transfer at the scan rate range from 20 to 500mVs-1. Based on the immobilization of cytochrome c on NaY zeolite a high performance biosensor was constructed, which displayed an excellent response to the reduction of hydrogen peroxide (H2O 2) without the aid of an electron mediator and could be used for H2O2 detection. NaY zeolite provided a good matrix for protein immobilization and biosensor preparation.

Original languageEnglish
Pages (from-to)2139-2144
Number of pages6
JournalElectrochimica Acta
Volume49
Issue number13
DOIs
StatePublished - 30 May 2004
Externally publishedYes

Keywords

  • Biosensors
  • Chemically modified electrodes
  • Cytochrome c
  • Direct electron transfer
  • Hydrogen peroxide
  • NaY zeolite

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