Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli

Min Jiang; Shu Wen Liu; Jiang Feng Ma; Ke Quan Chen; Li Yu; Fang Fang Yue; Bing Xu; Ping Wei

doi:10.1128/AEM.02190-09

Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli

Min Jiang, Shu Wen Liu, Jiang Feng Ma, Ke Quan Chen, Li Yu, Fang Fang Yue, Bing Xu, Ping Wei

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt snowed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.

Original language	English
Pages (from-to)	1298-1300
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	76
Issue number	4
DOIs	https://doi.org/10.1128/AEM.02190-09
State	Published - Feb 2010

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abstract = "Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt snowed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.",

author = "Min Jiang and Liu, {Shu Wen} and Ma, {Jiang Feng} and Chen, {Ke Quan} and Li Yu and Yue, {Fang Fang} and Bing Xu and Ping Wei",

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AU - Jiang, Min

AU - Liu, Shu Wen

AU - Ma, Jiang Feng

AU - Chen, Ke Quan

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AU - Yue, Fang Fang

AU - Xu, Bing

AU - Wei, Ping

PY - 2010/2

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AB - Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt snowed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.

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ER -

Effect of growth phase feeding strategies on succinate production by metabolically engineered Escherichia coli

Abstract

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