Effect of overexpression of nicotinic acid phosphoribosyl transferase on succinic acid production in Escherichia coli NZN111

Rongming Liu, Jiangfeng Ma, Liya Liang, Bing Xu, Guangming Wang, Min Zhang, Min Jiang

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Escherichia coli strain NZN111 is a promising candidate for the fermentative production of succinate. However, because lactate dehydrogenase and pyruvate formate lyase were inactivated in NZN111, this strain had an unbalanced NADH/NAD+ ratio and could not use glucose under anaerobic conditions. In this study, a recombinant strain E. coli NZN111/pTrc99a-pncB was constructed to overexpress the nicotinic acid phosphoribosyl transferase gene (pncB). Under anaerobic conditions with the addition of 0.5 mmol/L nicotinic acid and 0.3 mmol/L isopropyl beta-D-thiogalactopyranoside (IPTG), the specific nicotinic acid phosphoribosyl transferase (NAPRTase, EC 2.4.2.11) activity in the recombinant strain was 11-fold higher than that in E. coli NZN111, the concentration of NAD(H) was increased by 3.85-fold, especially the concentration of NAD+ was increased by 5.17-fold and NADH/NAD+ was decreased from 0.640 to 0.125. The recombinant strain regained the capability of growth and glucose utilization under anaerobic conditions.

Original languageEnglish
Pages (from-to)1438-1447
Number of pages10
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume27
Issue number10
StatePublished - Oct 2011

Keywords

  • Dual-phase fermentation
  • Escherichia coli NZN111
  • Nicotinic acid phosphoribosyl transferase
  • Succinic acid

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