Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

Jie Lü; Peihong Mao; Xiang Jin; Long Yu; Hanjie Ying

Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

Jie Lü, Peihong Mao, Xiang Jin, Long Yu, Hanjie Ying

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Bacillus subtilis Bac01 was mutated by 15 keV N⁺ ions of 1.5×10¹⁶ cm^-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.

Original language	English
Pages (from-to)	271-276
Number of pages	6
Journal	Nuclear Science and Techniques/Hewuli
Volume	20
Issue number	5
State	Published - 20 Oct 2009

Keywords

Activity
Bacillus subtilis
Endo-1,4-β-D-glucanase
Gene mutation
Ion irradiation

Cite this

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title = "Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01",

abstract = "Bacillus subtilis Bac01 was mutated by 15 keV N+ ions of 1.5×1016 cm-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.",

keywords = "Activity, Bacillus subtilis, Endo-1,4-β-D-glucanase, Gene mutation, Ion irradiation",

author = "Jie L{\"u} and Peihong Mao and Xiang Jin and Long Yu and Hanjie Ying",

year = "2009",

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TY - JOUR

T1 - Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

AU - Lü, Jie

AU - Mao, Peihong

AU - Jin, Xiang

AU - Yu, Long

AU - Ying, Hanjie

PY - 2009/10/20

Y1 - 2009/10/20

N2 - Bacillus subtilis Bac01 was mutated by 15 keV N+ ions of 1.5×1016 cm-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.

AB - Bacillus subtilis Bac01 was mutated by 15 keV N+ ions of 1.5×1016 cm-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.

KW - Activity

KW - Bacillus subtilis

KW - Endo-1,4-β-D-glucanase

KW - Gene mutation

KW - Ion irradiation

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M3 - 文章

AN - SCOPUS:84969627602

SN - 1001-8042

VL - 20

SP - 271

EP - 276

JO - Nuclear Science and Techniques/Hewuli

JF - Nuclear Science and Techniques/Hewuli

IS - 5

ER -

Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

Abstract

Keywords

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