Enhancement in ionic liquid tolerance of cellulase immobilized on PEGylated graphene oxide nanosheets: Application in saccharification of lignocellulose

Jiaxing Xu; Zhenhuan Sheng; Xinfeng Wang; Xiaoyan Liu; Jun Xia; Peng Xiong; Bingfang He

doi:10.1016/j.biortech.2015.10.070

Enhancement in ionic liquid tolerance of cellulase immobilized on PEGylated graphene oxide nanosheets: Application in saccharification of lignocellulose

Jiaxing Xu, Zhenhuan Sheng, Xinfeng Wang, Xiaoyan Liu, Jun Xia, Peng Xiong, Bingfang He

School of Pharmaceutical Sciences

Huaiyin Normal University

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

The objective of the present work was to improve ionic liquid (IL) tolerance of cellulase based on the exploration of functional nanoscale carriers for potential application in lignocellulosic biorefinery. PEGylated graphene oxide (GO) composite was successfully fabricated by chemical binding of 4-arm-PEG-NH₂ and GO and applied to the immobilization of cellulase. The PEGylated GO-Cellulase retained 61% of the initial activity in 25% (w/v) 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) while free cellulase only retained 2%. The IL stability was enhanced more than 30 times. The relatively minor change in K_m value (from 2.7 to 3.2mgmL^-1) after the immobilization suggested that PEGylated GO-Cellulase was capable of closely mimicking the performance of free enzyme. After treating rice straw with [Bmim][Cl] and dilution to a final IL concentration of 15% (w/v), the slurry was directly hydrolyzed using PEGylated GO-Cellulase without IL removing and a high hydrolysis rate of 87% was achieved.

Original language	English
Pages (from-to)	1060-1064
Number of pages	5
Journal	Bioresource Technology
Volume	200
DOIs	https://doi.org/10.1016/j.biortech.2015.10.070
State	Published - 1 Jan 2016

Keywords

Cellulase
Graphene oxide
Ionic liquid
Saccharification
Tolerance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2015.10.070

Cite this

@article{d4322873b3684553a0adebbbcd4db86a,

title = "Enhancement in ionic liquid tolerance of cellulase immobilized on PEGylated graphene oxide nanosheets: Application in saccharification of lignocellulose",

abstract = "The objective of the present work was to improve ionic liquid (IL) tolerance of cellulase based on the exploration of functional nanoscale carriers for potential application in lignocellulosic biorefinery. PEGylated graphene oxide (GO) composite was successfully fabricated by chemical binding of 4-arm-PEG-NH2 and GO and applied to the immobilization of cellulase. The PEGylated GO-Cellulase retained 61% of the initial activity in 25% (w/v) 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) while free cellulase only retained 2%. The IL stability was enhanced more than 30 times. The relatively minor change in Km value (from 2.7 to 3.2mgmL-1) after the immobilization suggested that PEGylated GO-Cellulase was capable of closely mimicking the performance of free enzyme. After treating rice straw with [Bmim][Cl] and dilution to a final IL concentration of 15% (w/v), the slurry was directly hydrolyzed using PEGylated GO-Cellulase without IL removing and a high hydrolysis rate of 87% was achieved.",

keywords = "Cellulase, Graphene oxide, Ionic liquid, Saccharification, Tolerance",

author = "Jiaxing Xu and Zhenhuan Sheng and Xinfeng Wang and Xiaoyan Liu and Jun Xia and Peng Xiong and Bingfang He",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.biortech.2015.10.070",

language = "英语",

volume = "200",

pages = "1060--1064",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Enhancement in ionic liquid tolerance of cellulase immobilized on PEGylated graphene oxide nanosheets

T2 - Application in saccharification of lignocellulose

AU - Xu, Jiaxing

AU - Sheng, Zhenhuan

AU - Wang, Xinfeng

AU - Liu, Xiaoyan

AU - Xia, Jun

AU - Xiong, Peng

AU - He, Bingfang

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The objective of the present work was to improve ionic liquid (IL) tolerance of cellulase based on the exploration of functional nanoscale carriers for potential application in lignocellulosic biorefinery. PEGylated graphene oxide (GO) composite was successfully fabricated by chemical binding of 4-arm-PEG-NH2 and GO and applied to the immobilization of cellulase. The PEGylated GO-Cellulase retained 61% of the initial activity in 25% (w/v) 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) while free cellulase only retained 2%. The IL stability was enhanced more than 30 times. The relatively minor change in Km value (from 2.7 to 3.2mgmL-1) after the immobilization suggested that PEGylated GO-Cellulase was capable of closely mimicking the performance of free enzyme. After treating rice straw with [Bmim][Cl] and dilution to a final IL concentration of 15% (w/v), the slurry was directly hydrolyzed using PEGylated GO-Cellulase without IL removing and a high hydrolysis rate of 87% was achieved.

AB - The objective of the present work was to improve ionic liquid (IL) tolerance of cellulase based on the exploration of functional nanoscale carriers for potential application in lignocellulosic biorefinery. PEGylated graphene oxide (GO) composite was successfully fabricated by chemical binding of 4-arm-PEG-NH2 and GO and applied to the immobilization of cellulase. The PEGylated GO-Cellulase retained 61% of the initial activity in 25% (w/v) 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]) while free cellulase only retained 2%. The IL stability was enhanced more than 30 times. The relatively minor change in Km value (from 2.7 to 3.2mgmL-1) after the immobilization suggested that PEGylated GO-Cellulase was capable of closely mimicking the performance of free enzyme. After treating rice straw with [Bmim][Cl] and dilution to a final IL concentration of 15% (w/v), the slurry was directly hydrolyzed using PEGylated GO-Cellulase without IL removing and a high hydrolysis rate of 87% was achieved.

KW - Cellulase

KW - Graphene oxide

KW - Ionic liquid

KW - Saccharification

KW - Tolerance

UR - http://www.scopus.com/inward/record.url?scp=84948123303&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2015.10.070

DO - 10.1016/j.biortech.2015.10.070

M3 - 文章

C2 - 26526093

AN - SCOPUS:84948123303

SN - 0960-8524

VL - 200

SP - 1060

EP - 1064

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Enhancement in ionic liquid tolerance of cellulase immobilized on PEGylated graphene oxide nanosheets: Application in saccharification of lignocellulose

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this