Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.

Shi Qing Han; Ping Kai Ouyang; Ping Wei; Yi Hu

doi:10.1007/s10529-006-9175-7

Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.

Shi Qing Han, Ping Kai Ouyang, Ping Wei, Yi Hu

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.

Original language	English
Pages (from-to)	1909-1912
Number of pages	4
Journal	Biotechnology Letters
Volume	28
Issue number	23
DOIs	https://doi.org/10.1007/s10529-006-9175-7
State	Published - Dec 2006

Keywords

Biotransformation
Common vetch
Cyanohydrin
Oxynitrilase
Vicia sativa L.

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10.1007/s10529-006-9175-7

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title = "Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.",

abstract = "The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.",

keywords = "Biotransformation, Common vetch, Cyanohydrin, Oxynitrilase, Vicia sativa L.",

author = "Han, {Shi Qing} and Ouyang, {Ping Kai} and Ping Wei and Yi Hu",

year = "2006",

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doi = "10.1007/s10529-006-9175-7",

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pages = "1909--1912",

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T1 - Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.

AU - Han, Shi Qing

AU - Ouyang, Ping Kai

AU - Wei, Ping

AU - Hu, Yi

PY - 2006/12

Y1 - 2006/12

N2 - The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.

AB - The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52-100% and 88-99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.

KW - Biotransformation

KW - Common vetch

KW - Cyanohydrin

KW - Oxynitrilase

KW - Vicia sativa L.

UR - http://www.scopus.com/inward/record.url?scp=33750986209&partnerID=8YFLogxK

U2 - 10.1007/s10529-006-9175-7

DO - 10.1007/s10529-006-9175-7

M3 - 文章

C2 - 17028782

AN - SCOPUS:33750986209

SN - 0141-5492

VL - 28

SP - 1909

EP - 1912

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 23

ER -

Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.

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Keywords

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