Abstract
The composite of C@SiO2 with gold nanoparticles (AuNPs-C@SiO2) was fabricated by layer-by-layer assembly technique and was used to fabricate a novel hydrogen peroxide (H2O2) biosensor. The composite was composed of a colloidal carbon sphere core with an average diameter of 200 nm and a porous silica shell with a uniform thickness of about 50 nm, and decorated with gold nanoparticles on the surface. The porous silica shell could promote the composite to show high bio-compatibility and chemical stability. The AuNPs-C@SiO2 composite combined with hemoglobin (Hb) was used to construct a novel biosensor for the determination of H2O2, displaying a wide linear range from 5.0 to 80 μM with a detection limit of 0.08 μM at 3σ. The Kmapp value for the biosensor was determined to be 71.49 μM.
Original language | English |
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Pages (from-to) | 323-326 |
Number of pages | 4 |
Journal | Electrochemistry Communications |
Volume | 11 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2009 |
Externally published | Yes |
Keywords
- AuNPs-C@SiO
- Biosensors
- Hemoglobin