TY - JOUR
T1 - Fabrication of non-destructive and enhanced electrochemiluminescence interface for reusable detection of cell-released dopamine
AU - Wang, Zhaoyin
AU - Wang, Xiaoying
AU - Zhu, Xiaoshu
AU - Lv, Jianlin
AU - Zhang, Jia
AU - Zhu, Qinshu
AU - Dai, Zhihui
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/4/15
Y1 - 2019/4/15
N2 - Quantification of cell-released dopamine (DA) is essential to understand various biological processes. However, concentration of DA outside living cells may increase and decrease in a short time. Thus, approaches used to detect cell-released DA should meet higher demands. Herein, a simple and green method is employed to in situ growth of gold nanoparticles on the surface of WS2 nanosheets. The defects of prepared Au-WS2 nanocomposites may accelerate electron transfer and facilitate the absorption of dissolved oxygen. As a result, Au-WS2 nanocomposites-enhanced electrochemiluminescence (ECL) is observed for the first time, and is further used to fabricate a non-destructive and label-free analytical interface. The ECL interface can selectively detect DA in a range of 5.0 nM–200 μM with a detection limit of 3.23 nM. Meanwhile, since the architecture on the ECL interface is not destroyed during the detection procedure, DA can be quantified continuously at least six times after one fabrication. In addition, the proposed ECL interface can accurately monitor the release of DA from PC12 cells manipulated by K+ and nifidipine, indicating great potential of this ECL sensor for surveying DA-related investigations.
AB - Quantification of cell-released dopamine (DA) is essential to understand various biological processes. However, concentration of DA outside living cells may increase and decrease in a short time. Thus, approaches used to detect cell-released DA should meet higher demands. Herein, a simple and green method is employed to in situ growth of gold nanoparticles on the surface of WS2 nanosheets. The defects of prepared Au-WS2 nanocomposites may accelerate electron transfer and facilitate the absorption of dissolved oxygen. As a result, Au-WS2 nanocomposites-enhanced electrochemiluminescence (ECL) is observed for the first time, and is further used to fabricate a non-destructive and label-free analytical interface. The ECL interface can selectively detect DA in a range of 5.0 nM–200 μM with a detection limit of 3.23 nM. Meanwhile, since the architecture on the ECL interface is not destroyed during the detection procedure, DA can be quantified continuously at least six times after one fabrication. In addition, the proposed ECL interface can accurately monitor the release of DA from PC12 cells manipulated by K+ and nifidipine, indicating great potential of this ECL sensor for surveying DA-related investigations.
KW - Au-WS nanocomposites
KW - Cell-released dopamine
KW - ECL interface
KW - Non-destruction
KW - Reusability
UR - http://www.scopus.com/inward/record.url?scp=85060293043&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.01.087
DO - 10.1016/j.snb.2019.01.087
M3 - 文章
AN - SCOPUS:85060293043
SN - 0925-4005
VL - 285
SP - 438
EP - 444
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -