TY - JOUR
T1 - Global analysis of metabolites in rat and human urine based on gas chromatography/time-of-flight mass spectrometry
AU - A, Jiye
AU - Huang, Qing
AU - Wang, Guangji
AU - Zha, Weibin
AU - Yan, Bei
AU - Ren, Hongcan
AU - Gu, Shenghua
AU - Zhang, Ying
AU - Zhang, Qi
AU - Shao, Feng
AU - Sheng, Longsheng
AU - Sun, Jianguo
PY - 2008/8/1
Y1 - 2008/8/1
N2 - Sediment in urine may contain low-molecular-weight compounds that should be included in the analysis. To date, no systematic investigation has addressed this issue. We investigated three primary factors that influence the extraction efficiency of metabolites during preparation of urine samples for metabolomic research: centrifugation, pH, and extraction solvents. Obtained with the use of gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) technique and principal component analysis (PCA), our results indicate that (1) conventional centrifugation causes an apparent loss of some metabolites, indicating that urine samples for metabolomic research should not be centrifuged before procedures are undertaken to recover the metabolites; (2) pH adjustment has a large impact on the recovery of metabolites and is therefore not encouraged; (3) with design of experiment analysis, methanol and water yield the optimal extraction efficiency. Differences between rat and human urine were observed and are discussed. Ninety-nine metabolites identified in rat and human urine are presented. An efficient protocol is proposed for the pretreatment of urine samples.
AB - Sediment in urine may contain low-molecular-weight compounds that should be included in the analysis. To date, no systematic investigation has addressed this issue. We investigated three primary factors that influence the extraction efficiency of metabolites during preparation of urine samples for metabolomic research: centrifugation, pH, and extraction solvents. Obtained with the use of gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) technique and principal component analysis (PCA), our results indicate that (1) conventional centrifugation causes an apparent loss of some metabolites, indicating that urine samples for metabolomic research should not be centrifuged before procedures are undertaken to recover the metabolites; (2) pH adjustment has a large impact on the recovery of metabolites and is therefore not encouraged; (3) with design of experiment analysis, methanol and water yield the optimal extraction efficiency. Differences between rat and human urine were observed and are discussed. Ninety-nine metabolites identified in rat and human urine are presented. An efficient protocol is proposed for the pretreatment of urine samples.
KW - Design of experiment
KW - Gas chromatography/time-of-flight mass spectrometry
KW - Metabolomics
KW - Principal component analysis
KW - Urine metabolites
UR - http://www.scopus.com/inward/record.url?scp=50949107995&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2008.04.025
DO - 10.1016/j.ab.2008.04.025
M3 - 文章
C2 - 18486586
AN - SCOPUS:50949107995
SN - 0003-2697
VL - 379
SP - 20
EP - 26
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -