Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

Bin Zou; Yi Hu; Dinghua Yu; Jiaojiao Xia; Susu Tang; Weiming Liu; He Huang

doi:10.1016/j.bej.2010.09.005

Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

Bin Zou, Yi Hu, Dinghua Yu, Jiaojiao Xia, Susu Tang, Weiming Liu, He Huang

School of Pharmaceutical Sciences

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.

Original language	English
Pages (from-to)	150-153
Number of pages	4
Journal	Biochemical Engineering Journal
Volume	53
Issue number	1
DOIs	https://doi.org/10.1016/j.bej.2010.09.005
State	Published - 15 Dec 2010

Keywords

Hydrolysis
Immobilization
Ionic liquid
Lipase
Mesoporous material
Modification

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10.1016/j.bej.2010.09.005

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abstract = "Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.",

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AU - Zou, Bin

AU - Hu, Yi

AU - Yu, Dinghua

AU - Xia, Jiaojiao

AU - Tang, Susu

AU - Liu, Weiming

AU - Huang, He

PY - 2010/12/15

Y1 - 2010/12/15

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AB - Ionic liquid was synthesized and grafted onto the surface hydroxyl of mesoporous silica SBA-15 (IL-SBA). Porcine pancreas lipase (PPL) was successfully incorporated into the ionic liquid modified SBA-15 material by physical adsorption (PPL-IL-SBA). The enzymatic properties, including optimum temperature, pH and reusability of PPL-IL-SBA, were investigated in the triacetin hydrolysis reaction. Compared with parent SBA-15 immobilized lipase (PPL-SBA), the enzymatic properties have dramatically improved, especially the optimized activity increased from 594 to 975. U/g PPL after modification.

KW - Hydrolysis

KW - Immobilization

KW - Ionic liquid

KW - Lipase

KW - Mesoporous material

KW - Modification

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JO - Biochemical Engineering Journal

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Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica SBA-15

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Keywords

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