TY - JOUR
T1 - Kluyveromyces as promising yeast cell factories for industrial bioproduction
T2 - From bio-functional design to applications
AU - Qiu, Yibin
AU - Lei, Peng
AU - Wang, Rui
AU - Sun, Liang
AU - Luo, Zhengshan
AU - Li, Sha
AU - Xu, Hong
N1 - Publisher Copyright:
© 2023
PY - 2023/5/1
Y1 - 2023/5/1
N2 - As the two most widely used Kluyveromyces yeast, Kluyveromyces marxianus and K. lactis have gained increasing attention as microbial chassis in biocatalysts, biomanufacturing and the utilization of low-cost raw materials owing to their high suitability to these applications. However, due to slow progress in the development of molecular genetic manipulation tools and synthetic biology strategies, Kluyveromyces yeast cell factories as biological manufacturing platforms have not been fully developed. In this review, we provide a comprehensive overview of the attractive characteristics and applications of Kluyveromyces cell factories, with special emphasis on the development of molecular genetic manipulation tools and systems engineering strategies for synthetic biology. In addition, future avenues in the development of Kluyveromyces cell factories for the utilization of simple carbon compounds as substrates, the dynamic regulation of metabolic pathways, and for rapid directed evolution of robust strains are proposed. We expect that more synthetic systems, synthetic biology tools and metabolic engineering strategies will adapt to and optimize for Kluyveromyces cell factories to achieve green biofabrication of multiple products with higher efficiency.
AB - As the two most widely used Kluyveromyces yeast, Kluyveromyces marxianus and K. lactis have gained increasing attention as microbial chassis in biocatalysts, biomanufacturing and the utilization of low-cost raw materials owing to their high suitability to these applications. However, due to slow progress in the development of molecular genetic manipulation tools and synthetic biology strategies, Kluyveromyces yeast cell factories as biological manufacturing platforms have not been fully developed. In this review, we provide a comprehensive overview of the attractive characteristics and applications of Kluyveromyces cell factories, with special emphasis on the development of molecular genetic manipulation tools and systems engineering strategies for synthetic biology. In addition, future avenues in the development of Kluyveromyces cell factories for the utilization of simple carbon compounds as substrates, the dynamic regulation of metabolic pathways, and for rapid directed evolution of robust strains are proposed. We expect that more synthetic systems, synthetic biology tools and metabolic engineering strategies will adapt to and optimize for Kluyveromyces cell factories to achieve green biofabrication of multiple products with higher efficiency.
KW - Genetic toolkit
KW - Industrial application
KW - Kluyveromyces
KW - Renewable resource utilization
KW - Synthetic biology
KW - Yeast cell factory
UR - http://www.scopus.com/inward/record.url?scp=85150850879&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2023.108125
DO - 10.1016/j.biotechadv.2023.108125
M3 - 文献综述
C2 - 36870581
AN - SCOPUS:85150850879
SN - 0734-9750
VL - 64
JO - Biotechnology Advances
JF - Biotechnology Advances
M1 - 108125
ER -
