Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae

Zhongxue Dai; Honglian Gu; Shangjie Zhang; Fengxue Xin; Wenming Zhang; Weiliang Dong; Jiangfeng Ma; Honghua Jia; Min Jiang

doi:10.1016/j.biortech.2017.05.100

Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae

Zhongxue Dai, Honglian Gu, Shangjie Zhang, Fengxue Xin, Wenming Zhang, Weiliang Dong, Jiangfeng Ma, Honghua Jia, Min Jiang

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04 g/L consumption of methanol and 3.13% increase of cell growth (OD₆₀₀) when using methanol as the sole carbon source. Moreover, 0.26 g/L of pyruvate was detected in the fermentation broth. The supplementation of 1 g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35 g/L. This represents the first genetically modified non-methylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes.

Original language	English
Pages (from-to)	1407-1412
Number of pages	6
Journal	Bioresource Technology
Volume	245
DOIs	https://doi.org/10.1016/j.biortech.2017.05.100
State	Published - Dec 2017

Keywords

Methanol
Non-methylotrophy
Pyruvate
S. cerevisiae
Synthetic biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2017.05.100

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title = "Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae",

abstract = "The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04 g/L consumption of methanol and 3.13% increase of cell growth (OD600) when using methanol as the sole carbon source. Moreover, 0.26 g/L of pyruvate was detected in the fermentation broth. The supplementation of 1 g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35 g/L. This represents the first genetically modified non-methylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes.",

keywords = "Methanol, Non-methylotrophy, Pyruvate, S. cerevisiae, Synthetic biology",

author = "Zhongxue Dai and Honglian Gu and Shangjie Zhang and Fengxue Xin and Wenming Zhang and Weiliang Dong and Jiangfeng Ma and Honghua Jia and Min Jiang",

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year = "2017",

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doi = "10.1016/j.biortech.2017.05.100",

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T1 - Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae

AU - Dai, Zhongxue

AU - Gu, Honglian

AU - Zhang, Shangjie

AU - Xin, Fengxue

AU - Zhang, Wenming

AU - Dong, Weiliang

AU - Ma, Jiangfeng

AU - Jia, Honghua

AU - Jiang, Min

PY - 2017/12

Y1 - 2017/12

N2 - The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04 g/L consumption of methanol and 3.13% increase of cell growth (OD600) when using methanol as the sole carbon source. Moreover, 0.26 g/L of pyruvate was detected in the fermentation broth. The supplementation of 1 g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35 g/L. This represents the first genetically modified non-methylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes.

AB - The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04 g/L consumption of methanol and 3.13% increase of cell growth (OD600) when using methanol as the sole carbon source. Moreover, 0.26 g/L of pyruvate was detected in the fermentation broth. The supplementation of 1 g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35 g/L. This represents the first genetically modified non-methylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes.

KW - Methanol

KW - Non-methylotrophy

KW - Pyruvate

KW - S. cerevisiae

KW - Synthetic biology

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U2 - 10.1016/j.biortech.2017.05.100

DO - 10.1016/j.biortech.2017.05.100

M3 - 文章

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AN - SCOPUS:85019947047

SN - 0960-8524

VL - 245

SP - 1407

EP - 1412

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Metabolic construction strategies for direct methanol utilization in Saccharomyces cerevisiae

Abstract

Keywords

UN SDGs

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