Metabolic Engineering and Adaptive Evolution of Clostridium beijerinckii to Increase Solvent Production from Corn Stover Hydrolysate

Jinle Liu, Yu Jiang, Jun Chen, Junjie Yang, Weihong Jiang, Wei Zhuang, Hanjie Ying, Sheng Yang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The production of acetone-butanol-ethanol by solventogenic Clostridium using lignocellulosic biomass can be a potential alternative to petroleum-based butanol. However, previous studies on nondetoxified lignocellulose hydrolysate could not provide better results when compared to those in synthetic medium. In this study, we engineered the pentose pathway of Clostridium beijerinckii NCIMB 8052, which was then subjected to adaptive laboratory evolution in the gradient mixture of synthetic medium and pretreated corn stover enzymatic hydrolysate (CSH) prepared according to the National Renewable Energy Laboratory (NREL) standard. The final resultant strain CIBTS1274A produced 20.7 g/L of total solvents in NREL CSH diluted to 6% initial total sugars, supplemented with ammonium acetate. This performance was comparable with that of corn-based butanol. In addition, this strain was successfully used in the scale-up operation using nondetoxified corn stover and corncob hydrolysate at Lignicell Refining Biotechnologies Ltd., which once was the only commercial biobutanol industry in the world.

Original languageEnglish
Pages (from-to)7916-7925
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume68
Issue number30
DOIs
StatePublished - 29 Jul 2020

Keywords

  • Clostridium beijerinckii
  • adaptive evolution
  • butanol
  • lignocellulosic hydrolysate
  • pentose

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