Abstract
Changes in the cellular levels of glutathione (GSH) and protein S-glutathionylation (PSSG) are closely associated with a number of human diseases. Despite recent advances, few thiol-reactive, small-molecule GSH sensors could selectively detect GSH over other endogenous thiols, and none was capable of detecting PSSG in live mammalian cells. By using a dye-loaded mesoporous silica nanoquencher (qMSN) capped with anti-GSH antibody capable of highly selective binding toward GSH and glutathionylated proteins over other molecules, we have successfully developed a fluorescence GSH/PSSG nanosensor, which showed unprecedented selectivity toward PSSG even in the presence of GSH, had amplifiable and programmable fluorescence Turn-ON properties, and could be used to image endogenous PSSG in live mammalian cells under stimulated conditions for the first time.
Original language | English |
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Pages (from-to) | 10257-10262 |
Number of pages | 6 |
Journal | Angewandte Chemie - International Edition |
Volume | 57 |
Issue number | 32 |
DOIs | |
State | Published - 6 Aug 2018 |
Keywords
- antibodies
- biosensors
- glutathione
- glutathionylated proteins
- imaging
- mesoporous silica nanoquenchers