Nanoquencher-Based Selective Imaging of Protein Glutathionylation in Live Mammalian Cells

Xin Mao, Peiyan Yuan, Changmin Yu, Lin Li, Shao Q. Yao

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Changes in the cellular levels of glutathione (GSH) and protein S-glutathionylation (PSSG) are closely associated with a number of human diseases. Despite recent advances, few thiol-reactive, small-molecule GSH sensors could selectively detect GSH over other endogenous thiols, and none was capable of detecting PSSG in live mammalian cells. By using a dye-loaded mesoporous silica nanoquencher (qMSN) capped with anti-GSH antibody capable of highly selective binding toward GSH and glutathionylated proteins over other molecules, we have successfully developed a fluorescence GSH/PSSG nanosensor, which showed unprecedented selectivity toward PSSG even in the presence of GSH, had amplifiable and programmable fluorescence Turn-ON properties, and could be used to image endogenous PSSG in live mammalian cells under stimulated conditions for the first time.

Original languageEnglish
Pages (from-to)10257-10262
Number of pages6
JournalAngewandte Chemie - International Edition
Volume57
Issue number32
DOIs
StatePublished - 6 Aug 2018

Keywords

  • antibodies
  • biosensors
  • glutathione
  • glutathionylated proteins
  • imaging
  • mesoporous silica nanoquenchers

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