TY - JOUR
T1 - Nitrate removal, spatiotemporal communities of denitrifiers and the importance of their genetic potential for denitrification in novel denitrifying bioreactors
AU - Zhang, Yimin
AU - Wang, Longmian
AU - Han, Wei
AU - Wang, Xu
AU - Guo, Zhaobing
AU - Peng, Fuquan
AU - Yang, Fei
AU - Kong, Ming
AU - Gao, Yuexiang
AU - Chao, Jianying
AU - Wu, Dan
AU - Xu, Bin
AU - Zhu, Yueming
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017
Y1 - 2017
N2 - Nitrate treatment performance and denitrification activity were compared between denitrifying biological filters (DNBFs) based on dewatered alum sludge (DAS) and neutralized used acid (NUA). The spatiotemporal distribution of denitrifying genes and the genetic potential associated with denitrification activity and nitrate removal in both DNBFs were also evaluated. The removal efficiency of NUA–DNBF increased by 8% compared with that of DAS–DNBF, and the former NUA–DNBF emitted higher amount of N2O. Analysis of abundance and composition profiles showed that denitrifying gene patterns varied more or less in two matrices with different depths at three sampling times. Burkholderiales, Rhodocyclales, and Rhizobiales were the most commonly detected in both media during stable periods. Denitrification was determined by the abundance of specific genes or their ratios as revealed by controlling factors. The enhanced nitrate removal could be due to increasing qnosZ or decreasing ∑qnir/qnosZ. Furthermore, NUA–DNBF solely reduced nitrate by increasing the denitrification enzyme activity.
AB - Nitrate treatment performance and denitrification activity were compared between denitrifying biological filters (DNBFs) based on dewatered alum sludge (DAS) and neutralized used acid (NUA). The spatiotemporal distribution of denitrifying genes and the genetic potential associated with denitrification activity and nitrate removal in both DNBFs were also evaluated. The removal efficiency of NUA–DNBF increased by 8% compared with that of DAS–DNBF, and the former NUA–DNBF emitted higher amount of N2O. Analysis of abundance and composition profiles showed that denitrifying gene patterns varied more or less in two matrices with different depths at three sampling times. Burkholderiales, Rhodocyclales, and Rhizobiales were the most commonly detected in both media during stable periods. Denitrification was determined by the abundance of specific genes or their ratios as revealed by controlling factors. The enhanced nitrate removal could be due to increasing qnosZ or decreasing ∑qnir/qnosZ. Furthermore, NUA–DNBF solely reduced nitrate by increasing the denitrification enzyme activity.
KW - Denitrification enzyme activity
KW - Denitrifying biofilter
KW - Dewatered alum sludge
KW - Functional gene
KW - Neutralized used acid
UR - http://www.scopus.com/inward/record.url?scp=85020315741&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2017.05.205
DO - 10.1016/j.biortech.2017.05.205
M3 - 文章
C2 - 28601773
AN - SCOPUS:85020315741
SN - 0960-8524
VL - 241
SP - 552
EP - 562
JO - Bioresource Technology
JF - Bioresource Technology
ER -
