TY - JOUR
T1 - Novel electrochemical immune sensor based on Hep-PGA-PPy nanoparticles for detection of α-Fetoprotein in whole blood
AU - Xu, Tingting
AU - Chi, Bo
AU - Gao, Jian
AU - Chu, Meilin
AU - Fan, Wenlu
AU - Yi, Meihui
AU - Xu, Hong
AU - Mao, Chun
N1 - Publisher Copyright:
© 2017
PY - 2017/7/18
Y1 - 2017/7/18
N2 - A simple and accurate immune sensor for quantitative detection of α-Fetoprotein (AFP) was developed based on the immobilization of antigen on the surface of Hep-PGA-PPy nanoparticles modified glassy carbon electrodes (GCE). The obtained Hep-PGA-PPy nanoparticles were characterized by fourier transform infrared (FT-IR) spectra and transmission electron microscopy (TEM). And the blood compatibility of Hep-PGA-PPy nanoparticles was investigated by in vitro coagulation tests, hemolysis assay and whole blood adhesion tests. Combining the conductive property of polypyrrole (PPy) and the biocompatibility of heparin (Hep), the Hep-PGA-PPy nanoparticles could improve not only the anti-biofouling effect the electrode, but also improved the electrochemical properties of the immune sensor. Under optimal conditions, the proposed immune sensor could detect AFP in a linear range from 0.1 to 100 ng mL−1 with a detection limit of 0.099 ng mL−1 at the signal-to-noise ratio of 3, and it also possessed good reproducibility and storage stability. Furthermore, the detection of AFP in five human blood samples also showed satisfactory accuracy with low relative errors. Thus, the developed immune sensor which showed acceptable reproducibility, selectivity, stability and accuracy could be potentially used for the detection of whole blood samples directly.
AB - A simple and accurate immune sensor for quantitative detection of α-Fetoprotein (AFP) was developed based on the immobilization of antigen on the surface of Hep-PGA-PPy nanoparticles modified glassy carbon electrodes (GCE). The obtained Hep-PGA-PPy nanoparticles were characterized by fourier transform infrared (FT-IR) spectra and transmission electron microscopy (TEM). And the blood compatibility of Hep-PGA-PPy nanoparticles was investigated by in vitro coagulation tests, hemolysis assay and whole blood adhesion tests. Combining the conductive property of polypyrrole (PPy) and the biocompatibility of heparin (Hep), the Hep-PGA-PPy nanoparticles could improve not only the anti-biofouling effect the electrode, but also improved the electrochemical properties of the immune sensor. Under optimal conditions, the proposed immune sensor could detect AFP in a linear range from 0.1 to 100 ng mL−1 with a detection limit of 0.099 ng mL−1 at the signal-to-noise ratio of 3, and it also possessed good reproducibility and storage stability. Furthermore, the detection of AFP in five human blood samples also showed satisfactory accuracy with low relative errors. Thus, the developed immune sensor which showed acceptable reproducibility, selectivity, stability and accuracy could be potentially used for the detection of whole blood samples directly.
KW - Anti-biofouling
KW - Electrochemical immune sensor
KW - Hep-PGA-PPy nanoparticles
KW - Label-free
KW - α-Fetoprotein
UR - http://www.scopus.com/inward/record.url?scp=85018921407&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2017.04.045
DO - 10.1016/j.aca.2017.04.045
M3 - 文章
C2 - 28577596
AN - SCOPUS:85018921407
SN - 0003-2670
VL - 977
SP - 36
EP - 43
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -