Abstract
N-acety-L-ornithine deacetylase (NAOase) expressed by recombinant engineering strain BL21/pET22b-argE can be used to separate chiral aliphatic amino acids, and its optimal substrate is N-acetyl-D, L-methionine. In order to obtain the appropriate conversion conditions of N-acetyl-D, L-methionine by NAOase, the effects of temperature, pH, concentration of Co2+, concentration of substrate and enzyme dosage on conversion of N-acetyl-D, L-methionine were investigated. The results showed that the conversion of N-acetyl-D, L-methionine was 81.4% under the appropriate conditions of 37°C, pH7.0, concentration of Co2+1 mmol/L, reaction time 20 min, concentration of substrate 150 mmol/L and enzyme 5 g/L.
Original language | English |
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Pages (from-to) | 329-333 |
Number of pages | 5 |
Journal | Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology |
Volume | 25 |
Issue number | 4 |
State | Published - Aug 2009 |
Keywords
- Enzymatic resolution
- N-acety-L-ornithine deacetylase
- N-acetyl-D, L-methionine
- Operation condition