Preparation of L-phenylalanine by an aqueous two-phase system

Hua Zhou; Zhong Yao; Guanghai Sun; Ping Wei

Preparation of L-phenylalanine by an aqueous two-phase system

Hua Zhou, Zhong Yao, Guanghai Sun, Ping Wei

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The bioconversion of phenylpyruvate to L-phenylalanine using aspartate transaminase in an aqueous two-phase system (ATPS) was investigated. The optimal composition of an aqueous two-phase system was 20% of PEG4000 with 20% of Na₂HPO₄. Partition coefficients of phenylpyruvate, aspartate transaminase and L-phenylalanine respectively were 8.03, 31.7 and 0.74 in ATPS. The enzymatic yield of L-phenylalanine in ATPS was 78.1%, increased 27.4% higher than in aqueous system when the substrate concentration was 43.4 g/L.

Original language	English
Pages (from-to)	146-150
Number of pages	5
Journal	Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology
Volume	22
Issue number	2
State	Published - Apr 2006

Keywords

Aqueous two-phase system
Bioconversion
L-phenylalanine
Partition coefficient

Cite this

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abstract = "The bioconversion of phenylpyruvate to L-phenylalanine using aspartate transaminase in an aqueous two-phase system (ATPS) was investigated. The optimal composition of an aqueous two-phase system was 20% of PEG4000 with 20% of Na2HPO4. Partition coefficients of phenylpyruvate, aspartate transaminase and L-phenylalanine respectively were 8.03, 31.7 and 0.74 in ATPS. The enzymatic yield of L-phenylalanine in ATPS was 78.1%, increased 27.4% higher than in aqueous system when the substrate concentration was 43.4 g/L.",

keywords = "Aqueous two-phase system, Bioconversion, L-phenylalanine, Partition coefficient",

author = "Hua Zhou and Zhong Yao and Guanghai Sun and Ping Wei",

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T1 - Preparation of L-phenylalanine by an aqueous two-phase system

AU - Zhou, Hua

AU - Yao, Zhong

AU - Sun, Guanghai

AU - Wei, Ping

PY - 2006/4

Y1 - 2006/4

N2 - The bioconversion of phenylpyruvate to L-phenylalanine using aspartate transaminase in an aqueous two-phase system (ATPS) was investigated. The optimal composition of an aqueous two-phase system was 20% of PEG4000 with 20% of Na2HPO4. Partition coefficients of phenylpyruvate, aspartate transaminase and L-phenylalanine respectively were 8.03, 31.7 and 0.74 in ATPS. The enzymatic yield of L-phenylalanine in ATPS was 78.1%, increased 27.4% higher than in aqueous system when the substrate concentration was 43.4 g/L.

AB - The bioconversion of phenylpyruvate to L-phenylalanine using aspartate transaminase in an aqueous two-phase system (ATPS) was investigated. The optimal composition of an aqueous two-phase system was 20% of PEG4000 with 20% of Na2HPO4. Partition coefficients of phenylpyruvate, aspartate transaminase and L-phenylalanine respectively were 8.03, 31.7 and 0.74 in ATPS. The enzymatic yield of L-phenylalanine in ATPS was 78.1%, increased 27.4% higher than in aqueous system when the substrate concentration was 43.4 g/L.

KW - Aqueous two-phase system

KW - Bioconversion

KW - L-phenylalanine

KW - Partition coefficient

UR - http://www.scopus.com/inward/record.url?scp=33745740374&partnerID=8YFLogxK

M3 - 文章

AN - SCOPUS:33745740374

SN - 1001-7631

VL - 22

SP - 146

EP - 150

JO - Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology

JF - Huaxue Fanying Gongcheng Yu Gongyi/Chemical Reaction Engineering and Technology

IS - 2

ER -

Preparation of L-phenylalanine by an aqueous two-phase system

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Keywords

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