The De Novo Synthesis of 2-Phenylethanol from Glucose by the Synthetic Microbial Consortium Composed of Engineered Escherichia coli and Meyerozyma guilliermondii

Wei Yan, Hao Gao, Wankui Jiang, Yujia Jiang, Carol Sze Ki Lin, Wenming Zhang, Fengxue Xin, Min Jiang

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Synthetic microbial consortia show promising applications for fine chemical production, especially with long metabolic pathways. In this study, a synthetic microbial consortium consisting of Escherichia coli YLC20 and Meyerozyma guilliermondii MG57 was successfully constructed, which could achieve efficient de novo 2-phenylethanol (2-PE) production from glucose. A tyrosine-deficient E. coli YLC20 overexpressing genes of aroF and pheA was first constructed, which could accumulate 29.5 g/L of l-phenylalanine (l-Phe) within 96 h from glucose accompanied by the coproduction of acetate and α-ketoglutarate (α-KG). Furthermore, the engineered M. guilliermondii MG57 was constructed through the stepwise metabolic engineering strategy, which could facilitate the 2-PE synthesis from l-Phe. Moreover, the cosubstrate and material intervention strategies were applied to improve the stability of the microbial consortium and 2-PE production. Finally, the synthetic microbial consortium could de novo synthesize 3.77 g/L of 2-PE from 80 g/L of glucose, providing a reference for the de novo synthesis of fine chemicals with long metabolic pathways.

Original languageEnglish
Pages (from-to)4018-4030
Number of pages13
JournalACS Synthetic Biology
Volume11
Issue number12
DOIs
StatePublished - 16 Dec 2022

Keywords

  • 2-phenylethanol
  • de novo synthesis
  • interaction mechanism
  • microbial consortium
  • stability

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