TY - JOUR
T1 - Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer
AU - Liu, Yongxin
AU - Cao, Mengda
AU - Huang, Zhongxi
AU - Yu, Changmin
AU - Yang, Naidi
AU - Wu, Qiong
AU - Shi, Liang
AU - Duan, Wenjie
AU - Zhu, Yi
AU - Wei, Jifu
AU - Li, Lin
AU - Huang, Wei
N1 - Publisher Copyright:
© 2021
PY - 2022/4
Y1 - 2022/4
N2 - Rapid and accurate detection of immunoglobulin E (IgE) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of IgE from patient serum based on Fe3O4@SiO2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase (HRP)-labeled antibodies linked gold nanoparticles (AuNPs) as chemiluminescence (CL) signal amplifier for ultrasensitive detection of total IgE. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 kU/L, which is lowest in comparison with current methods, and far lower than that of ImmunoCAP for IgE detection (0.1 kU/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the ImmunoCAP for the quantitative detection of IgE, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the IgE traces in breast cancer patients, which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various IgE-related diseases.
AB - Rapid and accurate detection of immunoglobulin E (IgE) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of IgE from patient serum based on Fe3O4@SiO2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase (HRP)-labeled antibodies linked gold nanoparticles (AuNPs) as chemiluminescence (CL) signal amplifier for ultrasensitive detection of total IgE. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 kU/L, which is lowest in comparison with current methods, and far lower than that of ImmunoCAP for IgE detection (0.1 kU/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the ImmunoCAP for the quantitative detection of IgE, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the IgE traces in breast cancer patients, which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various IgE-related diseases.
KW - Cancer
KW - Gold nanoparticles
KW - IgE detection
KW - Magnetic nanocapturer
KW - Ultrasensitive immunosensor
UR - http://www.scopus.com/inward/record.url?scp=85117255235&partnerID=8YFLogxK
U2 - 10.1016/j.cclet.2021.08.117
DO - 10.1016/j.cclet.2021.08.117
M3 - 文章
AN - SCOPUS:85117255235
SN - 1001-8417
VL - 33
SP - 1855
EP - 1860
JO - Chinese Chemical Letters
JF - Chinese Chemical Letters
IS - 4
ER -
