一种新型分离传感膜的制备及其血糖的动态监测

Blood glucose is one of the most important physiological indicators. In clinical surgery, blood glucose can sensitively reflect the kidney function of patients to affect the postoperative healing and physiological. However, the existing clinical testing equipment, such as blood glucose meters, biochemical analyzers, etc., can only provide intermittent feedback of test results, and it is difficult to achieve dynamic monitoring of patients’blood sugar during surgery. As they can only collect intermittent feedback of detection results rely on discrete index points. In view of the above problems, this work proposed the combination of membrane separation technology and biosensing technology to construct a separation-sensing membrane which can synchronously realize dynamic separation of whole blood and online monitoring of blood glucose. Prussian blue (PB) nanoparticles and gold (Au) nanoparticles were grown in situ and synchronously on zirconia ceramic hollow fiber membrane (YSZ) via layer-by-layer self-assembly method. The effect of PB/Au on the hydrophilicity and hydrophobicity of the sensing membrane was investigated, and the plasma separation effect and blood glucose electrochemical detection performance were evaluated under the optimal membrane preparation conditions. The results showed that the best condition was 60 layers which can completely reject all the red blood cells, white blood cells and platelets in the whole blood. The separation-sensing membrane showed a sensitivity of 0.876 μA/(mmol/L), a liner range of 1—15 mmol/L to blood glucose and realized dynamic separation and detection in real human blood samples.