TY - JOUR
T1 - A novel ECL sensor for determination of carcinoembryonic antigen using reduced graphene Oxide-BaYF5:Yb, Er upconversion nanocomposites and gold nanoparticles
AU - Zhao, Linlin
AU - Li, Juan
AU - Liu, Yuhong
AU - Wei, Yuan
AU - Zhang, Jin
AU - Zhang, Jinzha
AU - Xia, Qing
AU - Zhang, Qiang
AU - Zhao, Wenbo
AU - Chen, Xiaoqiang
N1 - Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF5:Yb, Er (rGO-BaYF5:Yb, Er) nanocomposites. The rGO-BaYF5:Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV-vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF5:Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001-80 ng mL-1 with a low detection limit of 0.87 pg mL-1, good selectivity and satisfactory stability.
AB - We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF5:Yb, Er (rGO-BaYF5:Yb, Er) nanocomposites. The rGO-BaYF5:Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV-vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF5:Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001-80 ng mL-1 with a low detection limit of 0.87 pg mL-1, good selectivity and satisfactory stability.
KW - Carcinoembryonic antigen
KW - Electrochemiluminescence immunosensor
KW - Er nanocomposites
KW - Reduced graphene Oxide-BaYF:Yb
KW - Surface assembly
UR - http://www.scopus.com/inward/record.url?scp=84962732755&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2016.03.153
DO - 10.1016/j.snb.2016.03.153
M3 - 文章
AN - SCOPUS:84962732755
SN - 0925-4005
VL - 232
SP - 484
EP - 491
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -