Highly sensitive and portable aptasensor by using enzymatic nanoreactors as labels

Liting Yang, Yin Wang, Cheng Yao, Xuan Xu

科研成果: 期刊稿件文章同行评审

4 引用 (Scopus)

摘要

Due to the extremely low concentration of many small molecular targets, improving the sensitivity of aptasensors is urgently needed. Herein, a highly sensitive and portable biosensing platform of Fe3O4@Au-Apt/DMSNs-I-cDNA is designed on magnetic Fe3O4@Au by using enzymatic nanoreactors as signal labels. This signal label is prepared by encapsulating invertase into dendritic mesoporous silica nanoparticles (DMSNs-I), resulting in large enzyme load amount (407.3 ± 28 mg g−1) and high bioactivity recovery (78.56%). Therefore, DMSNs-I can efficiently convert sucrose into glucose with personal glucose meter (PGM) as readout. Under PGM assay mode, this sensing platform achieves accurate recognition and sensitive detection of aflatoxin B1 (AFB1) and bisphenol A (BPA) with low detection limits of 0.74 pg mL−1 and 0.094 pM (S/N = 3), respectively. Compared with the single invertase labelled aptasensor, the DMSNs-I labelled AFB1 aptasensor achieves ~3.19 times enhancement of sensitivity and 2 orders of magnitude lower in the detection limit. In addition, the proposed aptasensors are applied to detect AFB1 and BPA in complex samples with the acceptable recovery values. They also exhibited good selectivity and high stability, indicating the potential of the Fe3O4@Au-Apt/DMSNs-I-cDNA biosensing platform in constructing highly sensitive aptasensors for detecting small molecules.

源语言英语
文章编号106407
期刊Microchemical Journal
168
DOI
出版状态已出版 - 9月 2021

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