Photocontrollable fluorogenic probes for visualising near-membrane copper(II) in live cells

Buxiang Chen, Liulin Wang, Yanfei Zhao, Yun Ni, Chenqi Xin, Chengwu Zhang, Jinhua Liu, Jingyan Ge, Lin Li, Wei Huang

科研成果: 期刊稿件文章同行评审

13 引用 (Scopus)

摘要

In this work, we have described the design, synthesis, and characterisation of photocontrollable fluorogenic probe, Mem-5, which is equipped with a photolabile group (nitrobenzyl group), high brightness reporter (fluorescein), and membrane-anchoring unit (cholesterol or long aliphatic chain). This probe shows an intense fluorescence enhancement in response to copper(ii) without interference from 45 other analytes, including metal cations, amino acids, and anions, under biological conditions (pH 6-9). The hydrolysis reaction is second-order, with k = 9.01 × 10-5 M-1 s-1. The detection limit is 3.3 × 10-7 M in HEPES buffer (25 mM). The confocal fluorescence imaging results demonstrated that Mem-5 can visualise near-membrane copper(ii) in live mammalian cells. The clear advantage of our photocontrollable method is that it successfully avoids the influence of chemical species outside cells during near-membrane specific detection.

源语言英语
页(从-至)31093-31099
页数7
期刊RSC Advances
7
49
DOI
出版状态已出版 - 2017

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