TY - JOUR
T1 - Robust Controlled Degradation of Enzyme Loaded PCL-Based Fibrous Scaffolds Toward Scarless Skin Tissue Regeneration
AU - Fan, Lingling
AU - Dong, Weiliang
AU - Lu, Jianqi
AU - Peng, Yujia
AU - Xie, Bin
AU - Wei, Ping
AU - Jiang, Min
AU - Chen, Su
N1 - Publisher Copyright:
© 2025 The Author(s). Advanced Science published by Wiley-VCH GmbH.
PY - 2025
Y1 - 2025
N2 - Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on wound healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis of enzyme-loaded (Burkholderia cepacia lipase, BCL) polycaprolactone (PCL) nanofiber scaffolds, aligning with wound healing effects is reported. These scaffolds are fabricated via fiber microfluidic electrospinning degradation-control technique. The obtained scaffolds exhibit tunable degradation rates, achieving complete degradation within 12–72-h cycles. The acidic degradation products are further elucidated and reveal the potential degradation mechanism. The acidic degradation products create an optimal microenvironment during the hemostasis and inflammation stages of wound healing. Notably, in vivo experiments demonstrate the enzyme-loaded scaffolds effectively promote angiogenesis, reduce inflammatory responses, mitigate collagen deposition, and regulate fibroblast differentiation. This promotes rapid wound healing with a remarkable scarless rate of over 99% by day 21. New guidelines for scar-free healing dressings are proposed, which carry out faster degradation without microplastics (MPs) and toxic byproducts before scar formation. These principles might provide valuable insights and promise for developing more effective wound dressings.
AB - Uncontrolled degradation of wound dressings may result in residues, causing several negative effects on wound healing, such as secondary damage, undesirable inflammation, and scar skin formation. Here, an available strategy associated with the synthesis of enzyme-loaded (Burkholderia cepacia lipase, BCL) polycaprolactone (PCL) nanofiber scaffolds, aligning with wound healing effects is reported. These scaffolds are fabricated via fiber microfluidic electrospinning degradation-control technique. The obtained scaffolds exhibit tunable degradation rates, achieving complete degradation within 12–72-h cycles. The acidic degradation products are further elucidated and reveal the potential degradation mechanism. The acidic degradation products create an optimal microenvironment during the hemostasis and inflammation stages of wound healing. Notably, in vivo experiments demonstrate the enzyme-loaded scaffolds effectively promote angiogenesis, reduce inflammatory responses, mitigate collagen deposition, and regulate fibroblast differentiation. This promotes rapid wound healing with a remarkable scarless rate of over 99% by day 21. New guidelines for scar-free healing dressings are proposed, which carry out faster degradation without microplastics (MPs) and toxic byproducts before scar formation. These principles might provide valuable insights and promise for developing more effective wound dressings.
KW - degradation-control fibrous scaffolds
KW - enzyme-loaded wound dressings
KW - microplastics
KW - non-toxic by-products
KW - scarless skin tissue regeneration
UR - http://www.scopus.com/inward/record.url?scp=105002128419&partnerID=8YFLogxK
U2 - 10.1002/advs.202501053
DO - 10.1002/advs.202501053
M3 - 文章
AN - SCOPUS:105002128419
SN - 2198-3844
JO - Advanced Science
JF - Advanced Science
ER -