TY - JOUR
T1 - Signal-amplification and real-time fluorescence anisotropy detection of apyrase by carbon nanoparticle
AU - Liu, Jinhua
AU - Yu, Jing
AU - Chen, Jianrong
AU - Yang, Ronghua
AU - Shih, Kaimin
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Carbon nanomaterial combined with aptamer has been developed as an efficient bioanalytical method in sensor design. Herein, depending on carbon nanoparticle (cCNP)-enhanced fluorescence anisotropy (FA), a novel aptamer-based sensor (aptasensor) enabling signal-amplification and real-time detection of apyrase is reported. The foundation of our sensor design based on ATP-aptamer(P) can be adsorbed on the surface of cCNPs, resulting in the increase of FA due to the mass of cCNPs, and P-ATP complex has weak binding ability to cCNPs with minimal change of FA. Apyrase, being an integral membrane protein, can hydrolyze ATP and make P-ATP complex disassemble, and thus lead to the increasing of FA. Therefore, this approach is demonstrated to be a novel candidate for the detection of apyrase, with high sensitivity and selectivity. The linear dynamic range for the concentrations of apyrase is between 0.1 and 0.5 U/μL along with a detection limit of 0.05 U/μL. Furthermore, these results indicated that our design is a flexible and sensitive method for biomolecule analysis, which makes it promising for practical biomolecule analyses.
AB - Carbon nanomaterial combined with aptamer has been developed as an efficient bioanalytical method in sensor design. Herein, depending on carbon nanoparticle (cCNP)-enhanced fluorescence anisotropy (FA), a novel aptamer-based sensor (aptasensor) enabling signal-amplification and real-time detection of apyrase is reported. The foundation of our sensor design based on ATP-aptamer(P) can be adsorbed on the surface of cCNPs, resulting in the increase of FA due to the mass of cCNPs, and P-ATP complex has weak binding ability to cCNPs with minimal change of FA. Apyrase, being an integral membrane protein, can hydrolyze ATP and make P-ATP complex disassemble, and thus lead to the increasing of FA. Therefore, this approach is demonstrated to be a novel candidate for the detection of apyrase, with high sensitivity and selectivity. The linear dynamic range for the concentrations of apyrase is between 0.1 and 0.5 U/μL along with a detection limit of 0.05 U/μL. Furthermore, these results indicated that our design is a flexible and sensitive method for biomolecule analysis, which makes it promising for practical biomolecule analyses.
KW - Aptamer
KW - Apyrase
KW - Carbon nanoparticle
KW - Fluorescence anisotropy
KW - Sensor
UR - http://www.scopus.com/inward/record.url?scp=84894634591&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2014.02.001
DO - 10.1016/j.msec.2014.02.001
M3 - 文章
C2 - 24656370
AN - SCOPUS:84894634591
SN - 0928-4931
VL - 38
SP - 206
EP - 211
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 1
ER -