Abstract
A hydroxyphenylquinazolinone-based fluorescent probe DAP-1 with a large Stokes shift (162 nm) was firstly developed for detection of cysteine. The probe DAP-1 with two acrylate as highly Cys-selective sites was designed based on the combination of excited state intramolecular proton transfer (ESIPT) and aggregation-induced emission (AIE) mechanism. Upon the treatment with cysteine, DAP-1 displayed a strong fluorescence enhancement (65-fold). The limit of detection obtained from fluorescent titration was as low as 0.03 μM. DAP-1 could detect cysteine with high selectivity and sensitivity. Significantly, DAP-1 could be used to detect cysteine in living cells.
Original language | English |
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Pages (from-to) | 750-757 |
Number of pages | 8 |
Journal | Journal of Photochemistry and Photobiology A: Chemistry |
Volume | 364 |
DOIs | |
State | Published - 1 Sep 2018 |
Keywords
- Aggregation-induced emission
- Cell imaging
- Conjugate addition
- Cysteine
- Fluorescent probe