A hydroxyphenylquinazolinone-based fluorescent probe for turn-on detection of cysteine with a large Stokes shift and its application in living cells

Huace Sheng, Yonghong Hu, Yi Zhou, Shimin Fan, Yang Cao, Xinxin Zhao, Wenge Yang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A hydroxyphenylquinazolinone-based fluorescent probe DAP-1 with a large Stokes shift (162 nm) was firstly developed for detection of cysteine. The probe DAP-1 with two acrylate as highly Cys-selective sites was designed based on the combination of excited state intramolecular proton transfer (ESIPT) and aggregation-induced emission (AIE) mechanism. Upon the treatment with cysteine, DAP-1 displayed a strong fluorescence enhancement (65-fold). The limit of detection obtained from fluorescent titration was as low as 0.03 μM. DAP-1 could detect cysteine with high selectivity and sensitivity. Significantly, DAP-1 could be used to detect cysteine in living cells.

Original languageEnglish
Pages (from-to)750-757
Number of pages8
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume364
DOIs
StatePublished - 1 Sep 2018

Keywords

  • Aggregation-induced emission
  • Cell imaging
  • Conjugate addition
  • Cysteine
  • Fluorescent probe

Fingerprint

Dive into the research topics of 'A hydroxyphenylquinazolinone-based fluorescent probe for turn-on detection of cysteine with a large Stokes shift and its application in living cells'. Together they form a unique fingerprint.

Cite this