摘要
A hydroxyphenylquinazolinone-based fluorescent probe DAP-1 with a large Stokes shift (162 nm) was firstly developed for detection of cysteine. The probe DAP-1 with two acrylate as highly Cys-selective sites was designed based on the combination of excited state intramolecular proton transfer (ESIPT) and aggregation-induced emission (AIE) mechanism. Upon the treatment with cysteine, DAP-1 displayed a strong fluorescence enhancement (65-fold). The limit of detection obtained from fluorescent titration was as low as 0.03 μM. DAP-1 could detect cysteine with high selectivity and sensitivity. Significantly, DAP-1 could be used to detect cysteine in living cells.
源语言 | 英语 |
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页(从-至) | 750-757 |
页数 | 8 |
期刊 | Journal of Photochemistry and Photobiology A: Chemistry |
卷 | 364 |
DOI | |
出版状态 | 已出版 - 1 9月 2018 |