A sensitive and selective fluorescence probe based fluorescein for detection of hypochlorous acid and its application for biological imaging

Wenzhu Yin; Hongjun Zhu; Ruiyong Wang

doi:10.1016/j.dyepig.2014.03.012

A sensitive and selective fluorescence probe based fluorescein for detection of hypochlorous acid and its application for biological imaging

Wenzhu Yin, Hongjun Zhu, Ruiyong Wang

School of Chemistry and Moleculai Engineering

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

A highly sensitive and selective fluorescein-based probe 1 for hypochlorite anion was synthesized. The probe 1 has favorable characteristics for biological imaging, including high water solubility, high fluorescence yield, pH-independent fluorescence, and biocompatibility. Results show that it has a detection limit of 40 nM to hypochlorite anion. In addition, confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe 1 could be used as an effective fluorescent probe for detecting HOCl in living cells.

Original language	English
Pages (from-to)	127-132
Number of pages	6
Journal	Dyes and Pigments
Volume	107
DOIs	https://doi.org/10.1016/j.dyepig.2014.03.012
State	Published - Aug 2014

Keywords

Biological imaging
Flourescent probe
Fluorescein
Hypochlorite anion
Spirolactam
Water solubility

Access to Document

10.1016/j.dyepig.2014.03.012

Cite this

@article{52e1eaa2607948b48810b9dec68b1c26,

title = "A sensitive and selective fluorescence probe based fluorescein for detection of hypochlorous acid and its application for biological imaging",

abstract = "A highly sensitive and selective fluorescein-based probe 1 for hypochlorite anion was synthesized. The probe 1 has favorable characteristics for biological imaging, including high water solubility, high fluorescence yield, pH-independent fluorescence, and biocompatibility. Results show that it has a detection limit of 40 nM to hypochlorite anion. In addition, confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe 1 could be used as an effective fluorescent probe for detecting HOCl in living cells.",

keywords = "Biological imaging, Flourescent probe, Fluorescein, Hypochlorite anion, Spirolactam, Water solubility",

author = "Wenzhu Yin and Hongjun Zhu and Ruiyong Wang",

year = "2014",

month = aug,

doi = "10.1016/j.dyepig.2014.03.012",

language = "英语",

volume = "107",

pages = "127--132",

journal = "Dyes and Pigments",

issn = "0143-7208",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A sensitive and selective fluorescence probe based fluorescein for detection of hypochlorous acid and its application for biological imaging

AU - Yin, Wenzhu

AU - Zhu, Hongjun

AU - Wang, Ruiyong

PY - 2014/8

Y1 - 2014/8

N2 - A highly sensitive and selective fluorescein-based probe 1 for hypochlorite anion was synthesized. The probe 1 has favorable characteristics for biological imaging, including high water solubility, high fluorescence yield, pH-independent fluorescence, and biocompatibility. Results show that it has a detection limit of 40 nM to hypochlorite anion. In addition, confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe 1 could be used as an effective fluorescent probe for detecting HOCl in living cells.

AB - A highly sensitive and selective fluorescein-based probe 1 for hypochlorite anion was synthesized. The probe 1 has favorable characteristics for biological imaging, including high water solubility, high fluorescence yield, pH-independent fluorescence, and biocompatibility. Results show that it has a detection limit of 40 nM to hypochlorite anion. In addition, confocal fluorescence microscopy imaging using RAW264.7 cells showed that the new probe 1 could be used as an effective fluorescent probe for detecting HOCl in living cells.

KW - Biological imaging

KW - Flourescent probe

KW - Fluorescein

KW - Hypochlorite anion

KW - Spirolactam

KW - Water solubility

UR - http://www.scopus.com/inward/record.url?scp=84898964744&partnerID=8YFLogxK

U2 - 10.1016/j.dyepig.2014.03.012

DO - 10.1016/j.dyepig.2014.03.012

M3 - 文章

AN - SCOPUS:84898964744

SN - 0143-7208

VL - 107

SP - 127

EP - 132

JO - Dyes and Pigments

JF - Dyes and Pigments

ER -

A sensitive and selective fluorescence probe based fluorescein for detection of hypochlorous acid and its application for biological imaging

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this