Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

Xueli Ren; Jun Akimoto; Hideyuki Miyatake; Seiichi Tada; Liping Zhu; Hongli Mao; Takashi Isoshima; Stefan Müller; Seong Min Kim; Yulai Zhou; Yoshihiro Ito

doi:10.1039/c9tb00407f

Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

Xueli Ren, Jun Akimoto, Hideyuki Miyatake, Seiichi Tada, Liping Zhu, Hongli Mao, Takashi Isoshima, Stefan Müller, Seong Min Kim, Yulai Zhou, Yoshihiro Ito

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

Original language	English
Pages (from-to)	4272-4279
Number of pages	8
Journal	Journal of Materials Chemistry B
Volume	7
Issue number	27
DOIs	https://doi.org/10.1039/c9tb00407f
State	Published - 2019
Externally published	Yes

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title = "Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms",

abstract = "Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.",

author = "Xueli Ren and Jun Akimoto and Hideyuki Miyatake and Seiichi Tada and Liping Zhu and Hongli Mao and Takashi Isoshima and Stefan M{\"u}ller and Kim, {Seong Min} and Yulai Zhou and Yoshihiro Ito",

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year = "2019",

doi = "10.1039/c9tb00407f",

language = "英语",

volume = "7",

pages = "4272--4279",

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TY - JOUR

T1 - Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

AU - Ren, Xueli

AU - Akimoto, Jun

AU - Miyatake, Hideyuki

AU - Tada, Seiichi

AU - Zhu, Liping

AU - Mao, Hongli

AU - Isoshima, Takashi

AU - Müller, Stefan

AU - Kim, Seong Min

AU - Zhou, Yulai

AU - Ito, Yoshihiro

PY - 2019

Y1 - 2019

N2 - Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

AB - Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

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DO - 10.1039/c9tb00407f

M3 - 文章

AN - SCOPUS:85068736908

SN - 2050-750X

VL - 7

SP - 4272

EP - 4279

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 27

ER -

Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

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