Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

Xueli Ren; Jun Akimoto; Hideyuki Miyatake; Seiichi Tada; Liping Zhu; Hongli Mao; Takashi Isoshima; Stefan Müller; Seong Min Kim; Yulai Zhou; Yoshihiro Ito

doi:10.1039/c9tb00407f

Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

Xueli Ren, Jun Akimoto, Hideyuki Miyatake, Seiichi Tada, Liping Zhu, Hongli Mao, Takashi Isoshima, Stefan Müller, Seong Min Kim, Yulai Zhou, Yoshihiro Ito

科研成果: 期刊稿件 › 文章 › 同行评审

10 引用（Scopus）

摘要

Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

源语言	英语
页（从-至）	4272-4279
页数	8
期刊	Journal of Materials Chemistry B
卷	7
期	27
DOI	https://doi.org/10.1039/c9tb00407f
出版状态	已出版 - 2019
已对外发布	是

访问文件

10.1039/c9tb00407f

其它文件与链接

链接到 Scopus 的出版物

引用此

@article{2d89dc17a04f418a846c515caa17ff48,

title = "Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms",

abstract = "Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.",

author = "Xueli Ren and Jun Akimoto and Hideyuki Miyatake and Seiichi Tada and Liping Zhu and Hongli Mao and Takashi Isoshima and Stefan M{\"u}ller and Kim, {Seong Min} and Yulai Zhou and Yoshihiro Ito",

note = "Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/c9tb00407f",

language = "英语",

volume = "7",

pages = "4272--4279",

journal = "Journal of Materials Chemistry B",

issn = "2050-750X",

publisher = "Royal Society of Chemistry",

number = "27",

}

TY - JOUR

T1 - Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

AU - Ren, Xueli

AU - Akimoto, Jun

AU - Miyatake, Hideyuki

AU - Tada, Seiichi

AU - Zhu, Liping

AU - Mao, Hongli

AU - Isoshima, Takashi

AU - Müller, Stefan

AU - Kim, Seong Min

AU - Zhou, Yulai

AU - Ito, Yoshihiro

PY - 2019

Y1 - 2019

N2 - Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

AB - Vascular endothelial growth factors (VEGFs) play an important role in tissue repair and regeneration. In this study, VEGFs were immobilised by photo-reactive gelatine by photo-irradiation and the effect of immobilisation was quantitatively investigated. Immobilised VEGFs enhanced endothelial cell migration and growth more than soluble VEGFs. In addition, the activation effects depended on the VEGF isoforms. Prolonged activation of phosphorylation sites in the VEGF receptor, which is associated with migration and proliferation, was observed on the immobilised VEGF surfaces. The results indicated that photo-immobilised VEGFs induced high cell activation by stimulating phosphorylation of the VEGF receptor, VEGFR2, which activated multiple cell functions.

UR - http://www.scopus.com/inward/record.url?scp=85068736908&partnerID=8YFLogxK

U2 - 10.1039/c9tb00407f

DO - 10.1039/c9tb00407f

M3 - 文章

AN - SCOPUS:85068736908

SN - 2050-750X

VL - 7

SP - 4272

EP - 4279

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 27

ER -

Cell migration and growth induced by photo-immobilised vascular endothelial growth factor (VEGF) isoforms

摘要

访问文件

其它文件与链接

指纹

引用此