Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles

Bin Zhou; Xueyu Yuan; Yonghong Hu; Jun Fan; Wenge Yang; Mingming Guo; Yi Zhang; Wenxin Li; Jue Zhang

doi:10.1080/09540105.2018.1548577

Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles

Bin Zhou, Xueyu Yuan, Yonghong Hu, Jun Fan, Wenge Yang, Mingming Guo, Yi Zhang, Wenxin Li, Jue Zhang

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal.

Original language	English
Pages (from-to)	87-94
Number of pages	8
Journal	Food and Agricultural Immunology
Volume	30
Issue number	1
DOIs	https://doi.org/10.1080/09540105.2018.1548577
State	Published - 1 Jan 2019

Keywords

Europium chelates
deoxynivalenol (DON)
magnetic nanoparticles

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10.1080/09540105.2018.1548577

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title = "Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles",

abstract = "Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal.",

keywords = "Europium chelates, deoxynivalenol (DON), magnetic nanoparticles",

author = "Bin Zhou and Xueyu Yuan and Yonghong Hu and Jun Fan and Wenge Yang and Mingming Guo and Yi Zhang and Wenxin Li and Jue Zhang",

note = "Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",

year = "2019",

month = jan,

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doi = "10.1080/09540105.2018.1548577",

language = "英语",

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T1 - Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles

AU - Zhou, Bin

AU - Yuan, Xueyu

AU - Hu, Yonghong

AU - Fan, Jun

AU - Yang, Wenge

AU - Guo, Mingming

AU - Zhang, Yi

AU - Li, Wenxin

AU - Zhang, Jue

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal.

AB - Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal.

KW - Europium chelates

KW - deoxynivalenol (DON)

KW - magnetic nanoparticles

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U2 - 10.1080/09540105.2018.1548577

DO - 10.1080/09540105.2018.1548577

M3 - 文章

AN - SCOPUS:85069443234

SN - 0954-0105

VL - 30

SP - 87

EP - 94

JO - Food and Agricultural Immunology

JF - Food and Agricultural Immunology

IS - 1

ER -

Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles

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Keywords

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