Detection of deoxynivalenol (DON) using europium chelates and magnetic nanoparticles

Bin Zhou, Xueyu Yuan, Yonghong Hu, Jun Fan, Wenge Yang, Mingming Guo, Yi Zhang, Wenxin Li, Jue Zhang

科研成果: 期刊稿件文章同行评审

9 引用 (Scopus)

摘要

Background: Several immunoassay methods have been developed for the detection of DON in cereal. However, there has been no method based on Eu-chelate antibody-conjugated magnetic nanoparticles established. Methods: Indirect competition for DON detection was established by combining Eu-chelate and immunomagnetic separation. The immunocomplex was separated and washed by exposure to a magnetic field and treatment with enhancement solution; then, fluorescences were measured according to the number of europium ions dissociated. Results: The working concentrations ranged from 0.05 to 100 ng/mL, and the sensitivity was 0.5 μg/Kg in cereal. The intra–assay CVs of corn and wheat were 5.7% and 7.1%, respectively. The recovery rates obtained using cereal sample were 83.2%–120.0%. Conclusion: This study suggested that the Eu-chelate antibody-conjugated magnetic nanoparticles method was fast and sensitive in screening large quantities of samples, and could be used for detection of DON in cereal.

源语言英语
页(从-至)87-94
页数8
期刊Food and Agricultural Immunology
30
1
DOI
出版状态已出版 - 1 1月 2019

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