Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Xiao Jun Ji; He Huang; Shuang Li; Jun Du; Min Lian

doi:10.1007/s10529-007-9599-8

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Xiao Jun Ji, He Huang, Shuang Li, Jun Du, Min Lian

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

Original language	English
Pages (from-to)	731-734
Number of pages	4
Journal	Biotechnology Letters
Volume	30
Issue number	4
DOIs	https://doi.org/10.1007/s10529-007-9599-8
State	Published - Apr 2008

Keywords

2,3-Butanediol
Klebsiella oxytoca
Proton-suicide method

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10529-007-9599-8

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title = "Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca",

abstract = "Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.",

keywords = "2,3-Butanediol, Klebsiella oxytoca, Proton-suicide method",

author = "Ji, {Xiao Jun} and He Huang and Shuang Li and Jun Du and Min Lian",

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T1 - Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

AU - Ji, Xiao Jun

AU - Huang, He

AU - Li, Shuang

AU - Du, Jun

AU - Lian, Min

PY - 2008/4

Y1 - 2008/4

N2 - Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

AB - Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

KW - 2,3-Butanediol

KW - Klebsiella oxytoca

KW - Proton-suicide method

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U2 - 10.1007/s10529-007-9599-8

DO - 10.1007/s10529-007-9599-8

M3 - 文章

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AN - SCOPUS:43149119974

SN - 0141-5492

VL - 30

SP - 731

EP - 734

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Abstract

Keywords

UN SDGs

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