Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Xiao Jun Ji; He Huang; Shuang Li; Jun Du; Min Lian

doi:10.1007/s10529-007-9599-8

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

Xiao Jun Ji, He Huang, Shuang Li, Jun Du, Min Lian

生物与制药工程学院

Nanjing Tech University

科研成果: 期刊稿件 › 文章 › 同行评审

79 引用（Scopus）

摘要

Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

源语言	英语
页（从-至）	731-734
页数	4
期刊	Biotechnology Letters
卷	30
期	4
DOI	https://doi.org/10.1007/s10529-007-9599-8
出版状态	已出版 - 4月 2008

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title = "Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca",

abstract = "Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.",

keywords = "2,3-Butanediol, Klebsiella oxytoca, Proton-suicide method",

author = "Ji, {Xiao Jun} and He Huang and Shuang Li and Jun Du and Min Lian",

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doi = "10.1007/s10529-007-9599-8",

language = "英语",

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T1 - Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

AU - Ji, Xiao Jun

AU - Huang, He

AU - Li, Shuang

AU - Du, Jun

AU - Lian, Min

PY - 2008/4

Y1 - 2008/4

N2 - Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

AB - Klebsiella oxytoca mutants were isolated from the wild type strain ME-303 after mutagenesis with UV coupled with diethyl sulfate and the following modified proton suicide method. By analyzing the activities of lactate dehydrogenase and phosphotransacetylase involved in lactic and acetic acid formation pathways and batch fermentation, one mutant, ME-UD-3, was isolated that produced 7.8% more 2,3-butanediol than ME-303 with the corresponding byproducts of lactic and acetic acid decreased by 88% and 92%, respectively.

KW - 2,3-Butanediol

KW - Klebsiella oxytoca

KW - Proton-suicide method

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U2 - 10.1007/s10529-007-9599-8

DO - 10.1007/s10529-007-9599-8

M3 - 文章

C2 - 18008166

AN - SCOPUS:43149119974

SN - 0141-5492

VL - 30

SP - 731

EP - 734

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -

Enhanced 2,3-butanediol production by altering the mixed acid fermentation pathway in Klebsiella oxytoca

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