Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux

Dong Liu; Yong Chen; An Li; Jingjing Xie; Jian Xiong; Jianxin Bai; Xiaochun Chen; Huanqing Niu; Tao Zhou; Hanjie Ying

doi:10.1007/s00449-011-0653-5

Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux

Dong Liu, Yong Chen, An Li, Jingjing Xie, Jian Xiong, Jianxin Bai, Xiaochun Chen, Huanqing Niu, Tao Zhou, Hanjie Ying

COLLEGE OF BIOTECHNOLOGY AND PHARMACEUTICAL ENGINEERING

Nanjing Tech University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH ₂PO ₄, MgCl ₂ and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH ₂PO ₄ 22.1 g/L; MgCl ₂ 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.

Original language	English
Pages (from-to)	729-737
Number of pages	9
Journal	Bioprocess and Biosystems Engineering
Volume	35
Issue number	5
DOIs	https://doi.org/10.1007/s00449-011-0653-5
State	Published - Jun 2012

Keywords

Metabolic flux analysis
Response surface method
Saccharomyces cerevisiae
Uridine 5′-monophosphate
Whole-cell biocatalysis

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title = "Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux",

abstract = "A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH 2PO 4, MgCl 2 and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH 2PO 4 22.1 g/L; MgCl 2 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.",

keywords = "Metabolic flux analysis, Response surface method, Saccharomyces cerevisiae, Uridine 5′-monophosphate, Whole-cell biocatalysis",

author = "Dong Liu and Yong Chen and An Li and Jingjing Xie and Jian Xiong and Jianxin Bai and Xiaochun Chen and Huanqing Niu and Tao Zhou and Hanjie Ying",

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doi = "10.1007/s00449-011-0653-5",

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journal = "Bioprocess and Biosystems Engineering",

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T1 - Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux

AU - Liu, Dong

AU - Chen, Yong

AU - Li, An

AU - Xie, Jingjing

AU - Xiong, Jian

AU - Bai, Jianxin

AU - Chen, Xiaochun

AU - Niu, Huanqing

AU - Zhou, Tao

AU - Ying, Hanjie

PY - 2012/6

Y1 - 2012/6

N2 - A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH 2PO 4, MgCl 2 and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH 2PO 4 22.1 g/L; MgCl 2 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.

AB - A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH 2PO 4, MgCl 2 and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH 2PO 4 22.1 g/L; MgCl 2 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.

KW - Metabolic flux analysis

KW - Response surface method

KW - Saccharomyces cerevisiae

KW - Uridine 5′-monophosphate

KW - Whole-cell biocatalysis

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U2 - 10.1007/s00449-011-0653-5

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AN - SCOPUS:84861481465

SN - 1615-7591

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JO - Bioprocess and Biosystems Engineering

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IS - 5

ER -

Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux

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Keywords

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