TY - JOUR
T1 - Enhanced uridine 5′-monophosphate production by whole cell of Saccharomyces cerevisiae through rational redistribution of metabolic flux
AU - Liu, Dong
AU - Chen, Yong
AU - Li, An
AU - Xie, Jingjing
AU - Xiong, Jian
AU - Bai, Jianxin
AU - Chen, Xiaochun
AU - Niu, Huanqing
AU - Zhou, Tao
AU - Ying, Hanjie
PY - 2012/6
Y1 - 2012/6
N2 - A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH 2PO 4, MgCl 2 and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH 2PO 4 22.1 g/L; MgCl 2 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.
AB - A whole-cell biocatalytic process for uridine 5′-monophosphate (UMP) production from orotic acid by Saccharomyces cerevisiae was developed. To rationally redistribute the metabolic flux between glycolysis and pentose phosphate pathway, statistical methods were employed first to find out the critical factors in the process. NaH 2PO 4, MgCl 2 and pH were found to be the important factors affecting UMP production significantly. The levels of these three factors required for the maximum production of UMP were determined: NaH 2PO 4 22.1 g/L; MgCl 2 2.55 g/L; pH 8.15. An enhancement of UMP production from 6.12 to 8.13 g/L was achieved. A significant redistribution of metabolic fluxes was observed and the underlying mechanism was discussed.
KW - Metabolic flux analysis
KW - Response surface method
KW - Saccharomyces cerevisiae
KW - Uridine 5′-monophosphate
KW - Whole-cell biocatalysis
UR - http://www.scopus.com/inward/record.url?scp=84861481465&partnerID=8YFLogxK
U2 - 10.1007/s00449-011-0653-5
DO - 10.1007/s00449-011-0653-5
M3 - 文章
C2 - 22081050
AN - SCOPUS:84861481465
SN - 1615-7591
VL - 35
SP - 729
EP - 737
JO - Bioprocess and Biosystems Engineering
JF - Bioprocess and Biosystems Engineering
IS - 5
ER -