Kinetic model of enzymatic synthesis of γ-D-glutamyl-L-tryptophan

Qian Wang, Zhong Yao, Minggang Wu, Zhijin Xun, Zhi Zhou, Hong Xu, Ping Wei

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A new model of enzymatic synthesis of γ-D-Glu-L-Trp (SCV-07) from D-glutamine and L-tryptophan was developed. The Km and Kcat of transpeptidation and enzymatic hydrolysis of product (presented as Km′ and Kcat′) were determined as Km = 5.11 mmol·L-1, Kcat = 3.92 mmol·min-1, Km′ = 2.31 mmol·L-1 and Kcat′ = 1.46 mmol·min-1. The sequential mechanism was used to describe the rate of transpeptidation, and the model parameters were optimized. The enzymatic synthesis of γ-D-glutamyl-L-tryptophan was investigated to verify the model. Good agreement between experimental data and calculations was obtained. The total relative errors of experimental data and calculations were less than 5%.

Original languageEnglish
Pages (from-to)431-436
Number of pages6
JournalHuagong Xuebao/CIESC Journal
Volume59
Issue number2
StatePublished - Feb 2008

Keywords

  • Kinetic model
  • Reaction mechanism
  • γ-D-Glu-L-Trp
  • γ-glutamyltranspeptidase

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