Abstract
A new model of enzymatic synthesis of γ-D-Glu-L-Trp (SCV-07) from D-glutamine and L-tryptophan was developed. The Km and Kcat of transpeptidation and enzymatic hydrolysis of product (presented as Km′ and Kcat′) were determined as Km = 5.11 mmol·L-1, Kcat = 3.92 mmol·min-1, Km′ = 2.31 mmol·L-1 and Kcat′ = 1.46 mmol·min-1. The sequential mechanism was used to describe the rate of transpeptidation, and the model parameters were optimized. The enzymatic synthesis of γ-D-glutamyl-L-tryptophan was investigated to verify the model. Good agreement between experimental data and calculations was obtained. The total relative errors of experimental data and calculations were less than 5%.
Original language | English |
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Pages (from-to) | 431-436 |
Number of pages | 6 |
Journal | Huagong Xuebao/CIESC Journal |
Volume | 59 |
Issue number | 2 |
State | Published - Feb 2008 |
Keywords
- Kinetic model
- Reaction mechanism
- γ-D-Glu-L-Trp
- γ-glutamyltranspeptidase